酿酒酵母I型蛋白磷酸酶的缺陷会激活纺锤体/动粒检查点。
Defects in Saccharomyces cerevisiae protein phosphatase type I activate the spindle/kinetochore checkpoint.
作者信息
Bloecher A, Tatchell K
机构信息
Department of Biochemistry and Molecular Biology, Louisiana State University Medical Center, Shreveport, Louisiana 71130, USA.
出版信息
Genes Dev. 1999 Mar 1;13(5):517-22. doi: 10.1101/gad.13.5.517.
Abstract
A conditional allele of type 1 protein phosphatase (glc7-129) in Saccharomyces cerevisiae causes first cycle arrest in G2/M, characterized by cells with a short spindle and high H1 kinase activity. Point-of-execution experiments indicate Glc7p function is required in G2/M just before anaphase for the completion of mitosis. Loss of the spindle/kinetochore checkpoint in glc7-129 cells abolishes the G2/M cell cycle arrest with a concomitant increase in chromosome loss and reduced viability. These results support a role for Glc7p in regulating kinetochore attachment to the spindle, an event monitored by the spindle/kinetochore checkpoint.
摘要
酿酒酵母中1型蛋白磷酸酶的条件性等位基因（glc7-129）导致细胞在G2/M期的首次周期停滞，其特征是细胞纺锤体短且H1激酶活性高。执行点实验表明，在有丝分裂完成前的G2/M期，Glc7p功能是必需的。glc7-129细胞中纺锤体/动粒检查点的丧失消除了G2/M期细胞周期停滞，同时染色体丢失增加且存活率降低。这些结果支持了Glc7p在调节动粒与纺锤体附着中的作用，这是一个由纺锤体/动粒检查点监测的事件。
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