Liu C, Liao S, Gao Z
Department of Pathology, Beijing Medical University.
Zhonghua Bing Li Xue Za Zhi. 1997 Jun;26(3):155-7.
To investigate the relationship between Epstein-Barr virus (EBV) and bcl-2 gene.
Sixty-four paraffin-embeded cases of Hodgkin's disease (HD) were studied. Immunohistochemistry was performed by using monoclonal antibody to latent membrane protein 1 (LMP-1) and bcl-2. In situ hybridization for EBV encoded small mRNA-EBER on 41 cases. Double staining combined in situ hybridization and immunohistochemistry was performed for dual labelling of EBER and bcl-2 in seven cases.
The positive rates of LMP-1 and EBER were 39% and 44%. All cases which were EBER positive, also express LMP-1. The positive rate of bcl-2 protein was 23%. Only seven of these cases were also LMP-1 positive.
All the results suggest that the bcl-2 expression in the R-S cells in this series did not correlate with EBV-positivity.
探讨爱泼斯坦-巴尔病毒（EBV）与bcl-2基因之间的关系。
研究64例霍奇金淋巴瘤（HD）石蜡包埋病例。采用抗潜伏膜蛋白1（LMP-1）和bcl-2单克隆抗体进行免疫组织化学检测。对41例病例进行EBV编码的小mRNA-EBER原位杂交。对7例病例进行原位杂交和免疫组织化学联合双重染色，以对EBER和bcl-2进行双标记。
LMP-1和EBER的阳性率分别为39%和44%。所有EBER阳性的病例也表达LMP-1。bcl-2蛋白的阳性率为23%。这些病例中只有7例LMP-1也呈阳性。
所有结果表明，该系列中R-S细胞中的bcl-2表达与EBV阳性无关。
