Curtis S, Wilkinson G W, Westmoreland D
Department of Medical Microbiology, University Hospital of Wales, Heath Park, Cardiff.
J Med Microbiol. 1998 Jan;47(1):91-4. doi: 10.1099/00222615-47-1-91.
An outbreak of acute keratoconjunctivitis in an ophthalmology department affected 15 patients and seven members of staff and necessitated temporary closure of the unit. Adenovirus (Ad) was isolated from eye swabs taken during the outbreak, but typing of the isolates by virus neutralisation assay proved unsatisfactory, wrongly assigning the isolate to serotype 10. A robust and rapid method for preparing microgram amounts of Ad DNA from infected cells was devised and used as the basis of a non-radioactive method for routine genotyping of adenovirus clinical isolates. All isolates associated with the outbreak, and one from a patient presenting at a nearby hospital during the outbreak, were found to have restriction endonuclease digestion patterns characteristic of Ad 37.
某眼科部门爆发的急性角结膜炎感染了15名患者和7名工作人员，该科室不得不暂时关闭。在疫情爆发期间采集的眼拭子中分离出了腺病毒（Ad），但通过病毒中和试验对分离株进行分型的结果并不理想，错误地将分离株归为10型。我们设计了一种从感染细胞中制备微克量Ad DNA的强大且快速的方法，并将其作为腺病毒临床分离株常规基因分型的非放射性方法的基础。发现与此次疫情相关的所有分离株，以及疫情期间在附近医院就诊的一名患者的一株分离株，都具有Ad 37特有的限制性内切酶消化模式。
