Fawcett P T, Gibney K M, Vinette K M
Immunology Laboratory, Department of Clinical Science, duPont Hospital for Children, Wilmington, Delaware.
J Clin Microbiol. 1999 Apr;37(4):1045-8. doi: 10.1128/JCM.37.4.1045-1048.1999.
Cultures of Helicobacter pylori obtained from the American Type Culture Collection (strain 43504) were grown as isolated colonies or lawns on blood agar plates and in broth culture with constant shaking. Examination of bacterial growth with Gram-stained fixed preparation and differential interference contrast microscopy on wet preparations revealed that bacteria grown on blood agar plates had a morphology consistent with that normally reported for H. pylori whereas bacteria from broth cultures had the morphologic appearance of Helicobacter heilmannii. Bacteria harvested from blood agar plates assumed an H. heilmannii-like morphology when transferred to broth cultures, and bacteria from broth cultures grew with morphology typical of H. pylori when grown on blood agar plates. Analysis by PCR of bacteria isolated from blood agar plates and broth cultures indicated that a single strain of bacteria (H. pylori) was responsible for both morphologies.
从美国模式培养物保藏中心（菌株43504）获得的幽门螺杆菌培养物，在血琼脂平板上以单个菌落或菌苔形式生长，并在肉汤培养基中持续振荡培养。通过革兰氏染色固定标本检查细菌生长情况，并在湿标本上用微分干涉相差显微镜观察，结果显示在血琼脂平板上生长的细菌形态与通常报道的幽门螺杆菌形态一致，而肉汤培养物中的细菌具有海氏螺杆菌的形态外观。从血琼脂平板收获的细菌转移到肉汤培养基中时呈现出海氏螺杆菌样形态，而肉汤培养物中的细菌在血琼脂平板上生长时则呈现出幽门螺杆菌典型的形态。对从血琼脂平板和肉汤培养物中分离出的细菌进行PCR分析表明，单一菌株（幽门螺杆菌）导致了两种形态。
