Riska P F, Su Y, Bardarov S, Freundlich L, Sarkis G, Hatfull G, Carrière C, Kumar V, Chan J, Jacobs W R
Department of Medicine, State University of New York Health Science Center at Brooklyn, Brooklyn, New York 11203, USA.
J Clin Microbiol. 1999 Apr;37(4):1144-9. doi: 10.1128/JCM.37.4.1144-1149.1999.
Detecting antibiotic resistance in Mycobacterium tuberculosis is becoming increasingly important with the global recognition of drug-resistant strains and their adverse impact on clinical outcomes. Current methods of susceptibility testing are either time-consuming or costly; rapid, reliable, simple, and inexpensive methods would be highly desirable, especially in the developing world where most tuberculosis is found. The luciferase reporter phage is a unique reagent well-suited for this purpose: upon infection with viable mycobacteria, it produces quantifiable light which is not observed in mycobacterial cells treated with active antimicrobials. In this report, we describe a modification of our original assay, which allows detection of the emitted light with a Polaroid film box designated the Bronx Box. The technique has been applied to 25 M. tuberculosis reference and clinical strains, and criteria are presented which allow rapid and simple discrimination among strains susceptible or resistant to isoniazid and rifampin, the major antituberculosis agents.
随着全球对耐药菌株的认识及其对临床结果的不利影响，检测结核分枝杆菌中的抗生素耐药性变得越来越重要。目前的药敏试验方法要么耗时，要么成本高昂；快速、可靠、简单且廉价的方法将非常受欢迎，特别是在发现大多数结核病的发展中国家。荧光素酶报告噬菌体是一种非常适合此目的的独特试剂：在感染活的分枝杆菌后，它会产生可量化的光，而在用活性抗菌药物处理的分枝杆菌细胞中则不会观察到这种光。在本报告中，我们描述了对我们原始检测方法的一种改进，该改进允许使用一个名为布朗克斯盒的宝丽来胶片盒检测发出的光。该技术已应用于25株结核分枝杆菌参考菌株和临床菌株，并提出了一些标准，这些标准可以快速、简单地区分对主要抗结核药物异烟肼和利福平敏感或耐药的菌株。
