Holecek M, Skalská H, Mráz J
Department of Physiology, Charles University, School of Medicine, Hradec Králové, Czech Republic.
Amino Acids. 1999;16(1):1-11. doi: 10.1007/BF01318880.
The aims of the present study were to assess the changes of individual plasma amino acid levels in relation (1) to the severity of liver damage and (2) to the process of liver recovery. Acute liver injury was induced by an intragastric administration of CCl4 diluted in olive oil in doses of 2, 4 and/or 6 g of CCl4 per kg b.w. The control rats received olive oil only. Animals were sacrificed at 16, 24, 48 and 96 hours after treatment. The severity of liver injury was assessed by histological examination, by changes in ALT and AST in the blood plasma and by changes in liver weight. Statistical analysis was carried by ANOVA, p < 0.05 was considered significant. The Spearman rank correlation coefficient was used as a measure of the degree of linear relationship between variable and dose. In the period of the development of acute liver damage, i.e. at 16 and 24 hours after treatment, an increase in blood plasma amino acid levels and positive correlations with the dose of CCl4 were observed for most individual amino acids. The only exception was arginine which decreased in a dose dependent manner. At a phase of liver recovery, i.e. at 48 and 96 hours after CCl4 treatment, the concentrations of some individual amino acids decreased below the control values. The negative correlation with the dose of CCl4 occurred for taurine and isoleucine (at 48 hours) and taurine, threonine, valine, methionine, isoleucine and leucine (at 96 hours).
本研究的目的是评估个体血浆氨基酸水平的变化，这些变化与以下两方面相关：（1）肝损伤的严重程度；（2）肝脏恢复过程。通过胃内给予溶解于橄榄油中的四氯化碳诱导急性肝损伤，剂量为每千克体重2克、4克和/或6克四氯化碳。对照大鼠仅给予橄榄油。在治疗后16小时、24小时、48小时和96小时处死动物。通过组织学检查、血浆中谷丙转氨酶（ALT）和谷草转氨酶（AST）的变化以及肝脏重量的变化来评估肝损伤的严重程度。采用方差分析进行统计分析，p < 0.05被认为具有统计学意义。Spearman等级相关系数用于衡量变量与剂量之间的线性关系程度。在急性肝损伤发展阶段，即治疗后16小时和24小时，大多数个体氨基酸的血浆水平升高，且与四氯化碳剂量呈正相关。唯一的例外是精氨酸，其呈剂量依赖性降低。在肝脏恢复阶段，即四氯化碳治疗后48小时和96小时，一些个体氨基酸的浓度降至对照值以下。牛磺酸和异亮氨酸（在48小时时）以及牛磺酸、苏氨酸、缬氨酸、蛋氨酸、异亮氨酸和亮氨酸（在96小时时）与四氯化碳剂量呈负相关。
