威氏伊蚊（双翅目：蚊科）对巴马森林病毒的实验性感染与传播
Experimental infection and transmission of Barmah Forest virus by Aedes vigilax (Diptera: Culicidae).
作者信息
Boyd A M, Kay B H
机构信息
Mosquito Control Laboratory, Queensland Institute of Medical Research, Brisbane, Australia.
出版信息
J Med Entomol. 1999 Mar;36(2):186-9. doi: 10.1093/jmedent/36.2.186.
Aedes vigilax (Skuse) mosquitoes colonized from Townsville, Queensland, Australia, were fed on blood containing Barmah Forest virus (BF) isolated from the same species. The colony was susceptible to infection, with an ID50 of 10(2.6) CCID50 per mosquito. Infection and transmission rates for mosquitoes fed 10(3.5) CCID50 virus per mosquito varied from 58 to 100% and 36 to 100%, respectively, between days 3 and 13 after infection. Titers in infected mosquitoes were high by 5 d after infection and did not vary substantially thereafter. Virus transmission to suckling mice occurred from 3 d after infection. This work establishes that Ae. vigilax is a competent vector of BF.
从澳大利亚昆士兰州汤斯维尔采集的维氏伊蚊（Skuse）蚊子，以含有从同一物种中分离出的巴马森林病毒（BF）的血液为食。该蚊群易受感染，每只蚊子的半数感染剂量（ID50）为10(2.6) 组织培养感染剂量50（CCID50）。在感染后第3天至第13天之间，每只蚊子喂食10(3.5) CCID50病毒时，蚊子的感染率和传播率分别为58%至100%和36%至100%。感染蚊子的病毒滴度在感染后5天就很高，此后没有显著变化。感染后3天病毒开始传播给乳鼠。这项研究证实维氏伊蚊是巴马森林病毒的有效传播媒介。
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