Gusils C, González S N, Oliver G
Centro de Referencia para Lactobacilos, Tucumán, Argentina.
Can J Microbiol. 1999 Dec;45(12):981-7. doi: 10.1139/w99-102.
The beneficial effect of lactobacilli has been attributed to their ability to colonize human and animal gastrointestinal tracts. In this work, adhesion assays with three lactobacillus strains and intestinal fragments obtained from chickens were assessed. Lactobacillus animalis and L. fermentum were able to adhere to three kinds of epithelial cells (crop, small and large intestines) with predominance to small intestine. Among the strains considered, L. fermentum subsp. cellobiosus showed the lowest and L. animalis the highest adhesion ability. Scanning electron microphotographs showing L. animalis and L. fermentum adhering to intestinal cells were obtained. The characterization of L. animalis adhesion indicated that lectin-like structure of this strain has glucose/mannose as specific sugars of binding. However, a calcium requirement was not observed. The adhesion of L. fermentum was reduced by addition of sialic acid or mannose (P < 0.01). These carbohydrates can be involved in the interaction between adhesin and epithelial surface. In this case, the dependence on bivalent cations was demonstrated. Lactobacillus fermentum was effective in reducing the attachment of Salmonella pullorum by 77%, while L. animalis was able to inhibit (90%, 88%, and 78%) the adhesion of S. pullorum, S. enteritidis, and S. gallinarum to host-specific epithelial fragments respectively. Our results from this in vitro model suggest that these lactobacilli are able to block the binding sites for Salmonella adhesion.
乳酸杆菌的有益作用归因于它们在人和动物胃肠道中定殖的能力。在这项研究中，评估了三种乳酸杆菌菌株与从鸡身上获取的肠道片段的黏附试验。动物源乳酸杆菌和发酵乳杆菌能够黏附于三种上皮细胞（嗉囊、小肠和大肠），其中以小肠为主。在所研究的菌株中，纤维二糖发酵乳杆菌亚种的黏附能力最低，动物源乳酸杆菌的黏附能力最高。获得了显示动物源乳酸杆菌和发酵乳杆菌黏附于肠道细胞的扫描电子显微镜照片。动物源乳酸杆菌黏附特性表明，该菌株的凝集素样结构以葡萄糖/甘露糖作为特异性结合糖。然而，未观察到对钙的需求。添加唾液酸或甘露糖可降低发酵乳杆菌的黏附（P < 0.01）。这些碳水化合物可能参与黏附素与上皮表面之间的相互作用。在这种情况下，证明了对二价阳离子的依赖性。发酵乳杆菌能有效减少鸡白痢沙门氏菌的附着达77%，而动物源乳酸杆菌能够分别抑制鸡白痢沙门氏菌、肠炎沙门氏菌和鸡伤寒沙门氏菌对宿主特异性上皮片段的黏附（分别为90%、88%和78%）。我们在这个体外模型中的结果表明，这些乳酸杆菌能够阻断沙门氏菌黏附的结合位点。
