Department of Basic Medicine, Anqing Medical College, Anqing 246052, P.R. China.
School of Life Sciences and Anhui Key Laboratory of Biodiversity Research and Ecological Protection in Southwest Anhui, Anqing Normal University, Anqing 246133, P.R. China.
Lett Appl Microbiol. 2023 Jan 23;76(1). doi: 10.1093/lambio/ovac041.
The efficacy of Lactobacillus as an antibiotic substitute has been investigated as one of the potential strategies to prevent Salmonella infection in poultry. The purpose of this study was to explore the antibacterial activity of Lactobacillus fermentum 1.2133 (Lact. fermentum 1.2133) against Salmonella pullorum CVCC533 (Salm. pullorum CVCC533) and its effect on chickens infected with Salm. pullorum CVCC533. Results showed that Lact. fermentans 1.2133 has antibacterial activity against Salm. pullorum CVCC533 and the cell-free fermentation supernatant of Lact. fermentum 1.2133 had a bactericidal effect on the bacteria in the Salm. pullorum CVCC533 biofilm by significantly reducing the number of Salmonella and aerobic bacteria in the chicken duodenum, ileum, and cecum, including Escherichia shigella (P < 0.05), improved the species abundance of Lactobacilli (P < 0.05). The damage to the chicken intestine by Salm. pullorum CVCC533 was reduced as the expression of avian beta-defensin 2 (AvBD2) mRNA in chicken small intestine was increased (P < 0.05). The results showed that Lact. fermentum 1.2133 had the potential to be a probiotic for poultry due to its regulation of intestinal AvBD2 mRNA as well as its intestinal flora.
乳酸菌作为抗生素替代品的功效已被研究作为预防家禽沙门氏菌感染的潜在策略之一。本研究旨在探讨植物乳杆菌 1.2133（Lact. fermentum 1.2133）对鸡白痢沙门氏菌 CVCC533（Salm. pullorum CVCC533）的抗菌活性及其对感染鸡白痢沙门氏菌 CVCC533 的影响。结果表明，植物乳杆菌 1.2133 对鸡白痢沙门氏菌 CVCC533 具有抗菌活性，植物乳杆菌 1.2133 的无细胞发酵上清液对鸡白痢沙门氏菌 CVCC533 生物膜中的细菌具有杀菌作用，显著降低了鸡十二指肠、空肠和盲肠中沙门氏菌和需氧菌的数量，包括志贺氏菌（P<0.05），提高了乳酸菌的物种丰度（P<0.05）。鸡白痢沙门氏菌 CVCC533 对鸡肠道的损伤减轻，鸡小肠中禽β防御素 2（AvBD2）mRNA 的表达增加（P<0.05）。结果表明，由于植物乳杆菌 1.2133 能调节肠道 AvBD2 mRNA 及其肠道菌群，因此具有成为家禽益生菌的潜力。
