Sfalanga A, Di Cello F, Mugnai L, Tegli S, Fani R, Surico G
Istituto di Patologia e Zoologia forestale e agraria, Università di Firenze, Florence, Italy.
Res Microbiol. 1999 Jan-Feb;150(1):45-59. doi: 10.1016/s0923-2508(99)80045-x.
A new Burkholderia strain (PVFi5A) which exhibits antagonism towards many bacterial and fungal plant pathogens has been partially characterised. This strain was isolated from the rhizosphere of tomato plants and was referred to the Burkholderia cepacia complex on the basis of cultural, morphological and biochemical characteristics, including determination of the 16S ribosomal DNA sequence and fatty acid profile. Strain PVFi5A is a Gram-negative, aerobic bacterium, oxidase- and catalase-positive, motile with a polar tuft of flagella, able to grow on a variety of media without producing diffusible pigments; it is avirulent to onion, able to grow at 41 degrees C and resistant to several antibiotic substances. Its fatty acid profile contains the hydroxy acids 18:1 20H, 14:0 3OH and 16:0 3OH, but not the hydroxy acids 16:0 2OH. The antagonistic activity of strain PVFi5A is due to its production of various, as yet unidentified, antimicrobial compounds, one or more of which may differ from those reported previously for certain 'B. cepacia' strains. The ability of PVFi5A to suppress the growth of important bacterial and fungal phytopathogens makes this strain a potential biocontrol agent.
一种对多种细菌和真菌性植物病原体具有拮抗作用的新型伯克霍尔德氏菌菌株（PVFi5A）已得到部分特征描述。该菌株从番茄植株根际分离得到，基于培养、形态和生化特征，包括16S核糖体DNA序列测定和脂肪酸谱分析，被归类于洋葱伯克霍尔德菌复合体。菌株PVFi5A是革兰氏阴性需氧菌，氧化酶和过氧化氢酶呈阳性，具极生鞭毛束，能运动，可在多种培养基上生长且不产生可扩散色素；对洋葱无毒，能在41摄氏度生长，对多种抗生素物质具有抗性。其脂肪酸谱含有羟基酸18:1 20H、14:0 3OH和16:0 3OH，但不含有羟基酸16:0 2OH。菌株PVFi5A的拮抗活性归因于其产生的多种尚未鉴定的抗菌化合物，其中一种或多种可能与先前报道的某些“洋葱伯克霍尔德菌”菌株的抗菌化合物不同。PVFi5A抑制重要细菌和真菌性植物病原体生长的能力使其成为一种潜在的生物防治剂。
