DNA的免疫学。V. DNA/抗DNA复合物的分析。
Immunology of DNA. V. Analysis of DNA/anti-DNA complexes.
作者信息
Aarden L A, De Groot E R, Lakmaker F
出版信息
J Immunol Methods. 1976;13(3-4):241-52. doi: 10.1016/0022-1759(76)90071-5.
DNA/anti-DNA complexes were studied by equilibrium density centrifugation in CsC1 and by zonal centrifugation in sucrose gradients. Complexes prepared of circular DNA preparations PM2 DNA or SV40 DNA with anti-DNA obtained from patients with systemic lupus erythematosus were found to be stable under the conditions of CsC1 equilibrium centrifugation. This enabled homogeneous complexes of DNA and anti-DNA of composition AgAb1, AgAb2, AgAb3 etc to be isolated. The stability of these complexes, their banding pattern in CsC1 gradients and their sedimentation behaviour suggest that anti-DNA binds to DNA in a monogamous bivalent way.
通过在氯化铯中进行平衡密度离心以及在蔗糖梯度中进行区带离心来研究DNA/抗DNA复合物。由环状DNA制剂PM2 DNA或SV40 DNA与从系统性红斑狼疮患者获得的抗DNA制备的复合物，在氯化铯平衡离心条件下被发现是稳定的。这使得能够分离出组成分别为AgAb1、AgAb2、AgAb3等的DNA和抗DNA的均匀复合物。这些复合物的稳定性、它们在氯化铯梯度中的条带模式以及它们的沉降行为表明，抗DNA以单配二价的方式与DNA结合。
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