Carr-Locke D L, Clayton P
Gut. 1978 Oct;19(10):916-22. doi: 10.1136/gut.19.10.916.
There is little information available on the bacteriological contamination of upper gastrointestinal fibreoptic endoscopes during routine use and the effects of 'disinfecting solutions'. A bacteriological evaluation was therefore made of cleaning an endoscope and its ancillary equipment with (1) water, (2) an aqueous solution of 1% cetrimide with 0.1% chlorhexidine, and (3) activated aqueous 2% glutaraldehyde. All equipment, but particularly the endoscope itself, was found to be heavily contaminated after use with a wide variety of organisms of which 53% were Gram positive. Cleaning the endoscope and ancillary equipment with water and the cetrimide/chlorhexidine solution alone or in combination was inadequate to produce disinfection but immersion in glutaraldehyde for two minutes consistently produced sterile cultures with our sampling technique. A rapid and simple method for disinfection of endoscopic equipment is therefore recommended and we think this is especially suitable for busy endoscopy units.
关于上消化道纤维内窥镜在常规使用过程中的细菌污染情况以及“消毒溶液”的效果，目前可获得的信息很少。因此，我们对用以下三种方法清洁内窥镜及其附属设备进行了细菌学评估：（1）水；（2）含0.1%洗必泰的1%溴化十六烷基三甲铵水溶液；（3）2%戊二醛活化水溶液。结果发现，所有设备，尤其是内窥镜本身，在使用后都被多种微生物严重污染，其中53%为革兰氏阳性菌。单独或联合用水以及溴化十六烷基三甲铵/洗必泰溶液清洁内窥镜及其附属设备，不足以达到消毒效果，但按照我们的采样技术，将其浸入戊二醛中两分钟能始终产生无菌培养物。因此，推荐一种快速简便的内窥镜设备消毒方法，我们认为这尤其适用于繁忙的内窥镜科室。
