St John K R, Zardiackas L D, Black R J, Armstrong R
University of Mississippi Medical Center, Department of Orthopaedic Surgery, Jackson 39216.
Clin Mater. 1993;12(1):49-55. doi: 10.1016/0267-6605(93)90027-5.
A model simulating a spiral diaphyseal fracture with butterfly fragments and bone loss was utilized to evaluate an hydroxyapatite/tricalcium phosphate, and collagen composite bone graft substitute in twelve dogs. The resultant grafted and contralateral control femora were tested in torsion at one year. This study examines the histological response to the graft material as well as crack propagation and fracture surface morphology using light microscopy and SEM. SEM and gross evaluation of the grafted bones revealed that 8/12 had fractured through bone outside the osteotomy site and all fractures included bone outside the graft site. No graft material was demonstrated at the points of initiation or termination of fracture for any of the bones. It was apparent that recorticalization had begun to occur at the graft site but the canal had not yet fully formed. The HA/TCP was seen to be tightly bound in tissue which had the appearance of new bone. Bone was found to be in direct apposition to the surface of the ceramic and within pores with no intervening soft tissue. Much of the new bone had remodeled into well organized Haversian systems with some patchy areas of woven bone and osteoid seen with polarized light illumination.
利用一个模拟伴有蝶形骨块和骨质缺损的螺旋形骨干骨折的模型，在12只犬身上评估一种羟基磷灰石/磷酸三钙和胶原蛋白复合骨移植替代物。对所得的移植侧股骨和对侧对照股骨在1年后进行扭转试验。本研究使用光学显微镜和扫描电子显微镜检查对移植材料的组织学反应以及裂纹扩展和骨折表面形态。扫描电子显微镜和对移植骨的大体评估显示，12例中有8例在截骨部位以外的骨质处发生骨折，所有骨折均累及移植部位以外的骨质。在任何一根骨头的骨折起始点或终止点均未发现移植材料。很明显，在移植部位已开始出现再皮质化，但髓腔尚未完全形成。可见羟基磷灰石/磷酸三钙紧密结合在具有新骨外观的组织中。发现骨直接贴附于陶瓷表面和孔隙内，其间无软组织。许多新骨已重塑为组织良好的哈弗斯系统，在偏振光照射下可见一些编织骨和类骨质的斑片状区域。
