Buimovici-Klein E, Vesikari T, Santangelo C F, Cooper L Z
Arch Virol. 1976;52(4):323-31. doi: 10.1007/BF01315621.
A whole blood culture method was used to study lymphocyte in vitro responses to rubella antigen and to phytohemagglutinin (PHA) in rubella infection. The acute phase of infection in four cases was characterized by high spontaneous incorporation of 14C-thymidine in the cultures, unresponsiveness of lymphocytes to rubella antigen, and absence of response, or relatively low response, to PHA. Cells showing vigorous in vitro response to rubella antigen appeared at about two weeks after the onset of rash. Lymphocyte PHA response returned to normal by day 31. Three rubella vaccinees exhibited a similar response. The use of whole blood lymphocyte cultures stimulated with multiple doses of mitogen and with antigen appears to be a promising technique for studies of "general" and "specific" cell-mediated immunity in viral infections.
采用全血培养法研究风疹感染时淋巴细胞对风疹抗原及植物血凝素（PHA）的体外反应。4例感染急性期的特点是培养物中14C-胸腺嘧啶核苷的自发掺入率高、淋巴细胞对风疹抗原无反应以及对PHA无反应或反应相对较低。出疹后约两周出现对风疹抗原体外反应强烈的细胞。淋巴细胞PHA反应在第31天时恢复正常。3名风疹疫苗接种者表现出类似反应。用多剂量有丝分裂原和抗原刺激的全血淋巴细胞培养法似乎是研究病毒感染中“一般”和“特异性”细胞介导免疫的一种有前景的技术。
