Shohat B, Shaltiel A, Ben-Bassat M, Joshua H
Cancer Lett. 1976 Nov;2(2):71-7. doi: 10.1016/s0304-3835(76)80014-6.
Mouse sarcoma 180 (S-180) solid and ascites tumor cells were treated in vivo and in vitro with withaferin A and observed with the electron microscope. The compound was found to effect this spindle microtubules of cells in metaphase. An interesting finding was the double membranes surrounding the chromosomes in the treated cells; probably the nuclei were reconstructed directly from the metaphase stage in the in vivo withaferin A-treated cells. In addition the membranes of the cells in interphase were affected by in vivo or in vitro treatment with the compound.
用穿心莲内酯对小鼠肉瘤180（S-180）实体瘤和腹水瘤细胞进行体内和体外处理，并用电镜观察。发现该化合物可作用于中期细胞的纺锤体微管。一个有趣的发现是，处理过的细胞中染色体周围有双层膜；可能在体内用穿心莲内酯处理的细胞中，细胞核是直接从中期阶段重建的。此外，化合物的体内或体外处理会影响间期细胞的膜。
