Stiernstedt S H, Tadesse T, Wretlind B
Karolinska Institute, Division of Infectious Diseases, Danderyd Hospital, Huddinge, Sweden.
APMIS. 1999 Apr;107(4):380-2. doi: 10.1111/j.1699-0463.1999.tb01568.x.
Minimal inhibitory concentration (MIC) of benzylpenicillin (Pen G) against strains of Borrelia burgdorferi has earlier been determined by a recently developed dialysis culture technique which provides a constant concentration of Pen G. To further investigate this method, both MIC and minimal bactericidal concentration (MBC) of Pen G against B. burgdorferi were determined and the results were compared with those obtained by other authors using different methods. The incubation period was 7 days and subcultures for MBC were observed for a further 2 weeks. The study showed results of MIC and MBC similar to those of broth microdilution, indicating sensitivity, but lower values than the majority of MIC and MBC results reported by other authors using broth macrodilution. It is essential for the results of antibiotic sensitivity testing in vitro against slowly growing bacteria like Borrelia burgdorferi that the concentration of antibiotics such as Pen G, which are unstable in solution, is constant during the incubation period.
苄青霉素（青霉素G）对伯氏疏螺旋体菌株的最低抑菌浓度（MIC）早前已通过一种最近开发的透析培养技术测定，该技术可提供恒定浓度的青霉素G。为进一步研究此方法，测定了青霉素G对伯氏疏螺旋体的MIC和最低杀菌浓度（MBC），并将结果与其他作者使用不同方法获得的结果进行比较。孵育期为7天，对MBC的传代培养再观察2周。该研究显示MIC和MBC结果与肉汤微量稀释法相似，表明具有敏感性，但低于其他作者使用肉汤常量稀释法报告的大多数MIC和MBC结果。对于体外针对像伯氏疏螺旋体这样生长缓慢的细菌进行抗生素敏感性测试的结果而言，像青霉素G这种在溶液中不稳定的抗生素在孵育期内浓度保持恒定至关重要。
