Steinbach G, Bahn P, Kreutzer B, Protz D, Staak C
Berl Munch Tierarztl Wochenschr. 1999 Apr;112(4):121-3.
According to the test protocol of the "meat juice ELISA" for detection of salmonella antibodies in pigs, all meat juice samples and serum controls are to be tested in duplicate. Results from routine investigations of repeatedly double tested meat juice and serum samples have been used to analyze the effect of double testing versus single testing with regard to the reliability of the final result. In case of an individual animal, testing of samples in duplicate increases the reliability of the results significantly, especially, if samples are retested at different occasions. In contrast, such a difference between mono and double testing of samples is not of importance when a group of animals is tested in order to determine the mean antibody rate in a herd. Here, results from double testing practically do not contribute to a higher reliability of the final result. This observation provides the possibility to reduce the costs for investigation programmes drastically.
根据用于检测猪沙门氏菌抗体的“肉汁ELISA”检测方案，所有肉汁样品和血清对照均需进行双份检测。对反复双份检测的肉汁和血清样品进行常规调查的结果，已用于分析双份检测与单份检测对最终结果可靠性的影响。对于单个动物，样品进行双份检测可显著提高结果的可靠性，特别是当样品在不同时间重新检测时。相比之下，当检测一组动物以确定畜群中的平均抗体率时，样品单份检测和双份检测之间的这种差异并不重要。在此情况下，双份检测的结果实际上对提高最终结果的可靠性并无帮助。这一观察结果为大幅降低调查项目成本提供了可能性。
