Poleksić V, Karan V
Faculty of Agriculture, University of Belgrade, Nemanjina 6, Beograd, YU-11080, Yugoslavia.
Ecotoxicol Environ Saf. 1999 Jun;43(2):213-21. doi: 10.1006/eesa.1999.1790.
Acute and subacute toxicity of the herbicide trifluralin on fish was investigated in laboratory toxicity tests with carp. Median lethal concentrations were determined in acute tests. The 96-h LC50 value was 0.045 (0.036-0.051)mg/L. Fish were exposed to subacute concentrations of the herbicide (0.005, 0.01, and 0.02 mg/L trifluralin) in the 14-day toxicity tests and the effects on the relative growth rate, some biochemical parameters [alkaline phosphatase (ALP), aspartate aminotransferase (AST), alanin aminotransferase (ALT) activities in serum, gills, liver, and kidney], gills, liver, and kidney structure were studied. A decrease in relative growth rate was found. An increase of functional enzyme activities in blood serum and the organs examined, particularly in the highest concentration of trifluralin indicated changes in the vital organs, was confirmed by histological analysis. The most severe changes (although mostly reversible) were found in the gills and kidney of the fish examined.
通过对鲤鱼进行实验室毒性试验，研究了除草剂氟乐灵对鱼类的急性和亚急性毒性。在急性试验中测定了半数致死浓度。96小时半数致死浓度（LC50）值为0.045（0.036 - 0.051）mg/L。在为期14天的毒性试验中，让鱼类接触亚急性浓度的除草剂（0.005、0.01和0.02 mg/L氟乐灵），并研究其对相对生长率、一些生化参数[血清、鳃、肝脏和肾脏中的碱性磷酸酶（ALP）、天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）活性]、鳃、肝脏和肾脏结构的影响。发现相对生长率下降。血清和所检查器官中功能酶活性增加，特别是在氟乐灵最高浓度组，这表明重要器官发生了变化，组织学分析证实了这一点。在所检查的鱼类的鳃和肾脏中发现了最严重的变化（尽管大多是可逆的）。
