酿酒酵母和卡尔斯伯酵母同源自主复制序列元件的DNA序列及功能分析
DNA sequence and functional analysis of homologous ARS elements of Saccharomyces cerevisiae and S. carlsbergensis.
作者信息
Theis J F, Yang C, Schaefer C B, Newlon C S
机构信息
Department of Microbiology and Molecular Genetics, New Jersey Medical School and Graduate School of Biomedical Sciences, University of Medicine and Dentistry of New Jersey, Newark, New Jersey 07103, USA.
出版信息
Genetics. 1999 Jul;152(3):943-52. doi: 10.1093/genetics/152.3.943.
ARS elements of Saccharomyces cerevisiae are the cis-acting sequences required for the initiation of chromosomal DNA replication. Comparisons of the DNA sequences of unrelated ARS elements from different regions of the genome have revealed no significant DNA sequence conservation. We have compared the sequences of seven pairs of homologous ARS elements from two Saccharomyces species, S. cerevisiae and S. carlsbergensis. In all but one case, the ARS308-ARS308(carl) pair, significant blocks of homology were detected. In the cases of ARS305, ARS307, and ARS309, previously identified functional elements were found to be conserved in their S. carlsbergensis homologs. Mutation of the conserved sequences in the S. carlsbergensis ARS elements revealed that the homologous sequences are required for function. These observations suggested that the sequences important for ARS function would be conserved in other ARS elements. Sequence comparisons aided in the identification of the essential matches to the ARS consensus sequence (ACS) of ARS304, ARS306, and ARS310(carl), though not of ARS310.
酿酒酵母的自主复制序列（ARS）元件是染色体DNA复制起始所需的顺式作用序列。对来自基因组不同区域的不相关ARS元件的DNA序列进行比较，未发现显著的DNA序列保守性。我们比较了来自酿酒酵母和卡尔斯伯酵母这两个酿酒酵母物种的七对同源ARS元件的序列。除了ARS308-ARS308（carl）这一对外，在所有其他情况下都检测到了显著的同源性区域。在ARS305、ARS307和ARS309的情况下，先前鉴定的功能元件在其卡尔斯伯酵母同源物中是保守的。卡尔斯伯酵母ARS元件中保守序列的突变表明同源序列是功能所必需的。这些观察结果表明，对ARS功能重要的序列在其他ARS元件中是保守的。序列比较有助于确定ARS304、ARS306和ARS310（carl）与ARS共有序列（ACS）的基本匹配，尽管ARS310没有。
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