对人类4号染色体q11 - q13区域进行物理和减数分裂图谱分析，该区域包含维生素D结合蛋白DBP/Gc球蛋白和白蛋白多基因簇。
Physical and meiotic mapping of the region of human chromosome 4q11-q13 encompassing the vitamin D binding protein DBP/Gc-globulin and albumin multigene cluster.
作者信息
Song Y H, Naumova A K, Liebhaber S A, Cooke N E
机构信息
Department of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104 USA.
出版信息
Genome Res. 1999 Jun;9(6):581-7.
The vitamin D binding protein/Gc-globulin (DBP) gene is a member of a multigene cluster that includes albumin (ALB), alpha-fetoprotein (AFP), and alpha-albumin/afamin (AFM). All four genes have structural and functional similarities and map to the same chromosomal regions in humans (4q11-q13), mice, and rats. An accurate physical map of the region encompassing these genes is a prerequisite for study of their respective transcriptional regulation and identification of potential shared regulatory elements. By refining the physical and meiotic maps of the 4q11-q13 region and creating a local PAC contig, the order and transcriptional orientations of these four genes were determined to be centromere-3'-DBP-5'-5'-ALB-3'-5'-AFP-3'-5'-AFM3'-telomere. The ancestral DBP gene was separated from the ALB gene by >1.5 Mb. This organization and spacing establishes a foundation for ongoing functional studies in this region.
维生素D结合蛋白/Gc球蛋白（DBP）基因是一个多基因簇的成员，该多基因簇包括白蛋白（ALB）、甲胎蛋白（AFP）和α-白蛋白/afamin（AFM）。这四个基因在结构和功能上具有相似性，并且在人类（4q11-q13）、小鼠和大鼠中定位到相同的染色体区域。包含这些基因的区域的精确物理图谱是研究它们各自的转录调控以及鉴定潜在共享调控元件的先决条件。通过完善4q11-q13区域的物理图谱和减数分裂图谱并创建一个局部PAC重叠群，确定这四个基因的顺序和转录方向为着丝粒-3'-DBP-5'-5'-ALB-3'-5'-AFP-3'-5'-AFM3'-端粒。祖先DBP基因与ALB基因相隔超过1.5 Mb。这种组织和间隔为该区域正在进行的功能研究奠定了基础。
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