Lass A, Forster M J, Sohal R S
Department of Biological Sciences, Southern Methodist University, Dallas, TX 75275, USA.
Free Radic Biol Med. 1999 Jun;26(11-12):1375-82. doi: 10.1016/s0891-5849(98)00330-x.
Coenzyme Q (CoQ) was previously demonstrated in vitro to indirectly act as an antioxidant in respiring mitochondria by regenerating alpha-tocopherol from its phenoxyl radical. The objective of this study was to determine whether CoQ has a similar sparing effect on alpha-tocopherol in vivo. Mice were administered CoQ10 (123 mg/kg/day) alone, or alpha-tocopherol (200 mg/kg/day) alone, or both, for 13 weeks, after which the amounts of CoQ10, CoQ9 and alpha-tocopherol were determined by HPLC in the serum as well as homogenates and mitochondria of liver, kidney, heart, upper hindlimb skeletal muscle and brain. Administration of CoQ10 and alpha-tocopherol, alone or together, increased the corresponding levels of CoQ10 and alpha-tocopherol in the serum. Supplementation with CoQ10 also elevated the amounts of the predominant homologue CoQ9 in the serum and the mitochondria. A notable effect of CoQ10 intake was the enhancement of alpha-tocopherol in mitochondria. alpha-Tocopherol administration resulted in an elevation of alpha-tocopherol content in the homogenates of nearly all tissues and their mitochondria. Results of this study thus indicate that relatively long-term administration of CoQ10 or alpha-tocopherol can result in an elevation of their concentrations in the tissues of the mouse. More importantly, CoQ10 intake has a sparing effect on alpha-tocopherol in mitochondria in vivo.
辅酶Q（CoQ）先前已在体外实验中被证明，通过将生育酚从其苯氧自由基中再生出来，从而在进行呼吸作用的线粒体中间接充当抗氧化剂。本研究的目的是确定CoQ在体内对生育酚是否具有类似的节约效应。给小鼠单独施用辅酶Q10（123毫克/千克/天），或单独施用生育酚（200毫克/千克/天），或两者同时施用，持续13周，之后通过高效液相色谱法测定血清以及肝脏、肾脏、心脏、后肢上部骨骼肌和大脑的匀浆及线粒体中辅酶Q10、辅酶Q9和生育酚的含量。单独或同时施用辅酶Q10和生育酚均可提高血清中相应的辅酶Q10和生育酚水平。补充辅酶Q10还可提高血清和线粒体中主要同系物辅酶Q9的含量。摄入辅酶Q10的一个显著作用是提高线粒体中的生育酚含量。施用生育酚可使几乎所有组织及其线粒体匀浆中的生育酚含量升高。因此，本研究结果表明，相对长期施用辅酶Q10或生育酚可导致小鼠组织中它们的浓度升高。更重要的是，摄入辅酶Q10在体内对线粒体中的生育酚具有节约效应。
