Sohal Rajindar S, Forster Michael J
Department of Pharmacology and Pharmaceutical Sciences, University of Southern California, 1985 Zonal Avenue, PSC 608, Los Angeles, CA 90089-9121, USA.
Mitochondrion. 2007 Jun;7 Suppl(Suppl):S103-11. doi: 10.1016/j.mito.2007.03.006. Epub 2007 Mar 30.
Coenzyme Q (CoQ) has three well-characterized functions in mitochondria, namely (i) transfer of reducing equivalents in the electron transport chain, (ii) generation of superoxide anion radical, O2*-, and (iii) quenching of free radicals. The main purpose of this review is to discuss the effects of CoQ10 intake for relatively prolonged periods on mitochondrial respiratory capacity, indicators of oxidative stress, and life span of animals, in context of the broader issue of whether or not the overall progression of the aging process can be modified by CoQ10 administration. Comparative studies on different mammalian species have indicated that the rate of mitochondrial superoxide anion radical generation is directly correlated with mitochondrial CoQ9 content and inversely related to amounts of CoQ10, particularly the CoQ10 bound to mitochondrial membrane proteins. Contrary to the historical view, dietary supplementation of mice and rats with CoQ10 has been demonstrated to augment the endogenous CoQ content (CoQ9 + CoQ10) in mitochondria and homogenates of various tissues, albeit to varying extent. Ingestion of CoQ10 results in the elevation of endogenous CoQ9, the predominant homologue in mice and rats. In our studies, there was no indication of a discernable effect of CoQ10 intake reflecting enhancement of mitochondrial respiratory activity, antioxidant capacity and pro-oxidant potentiation or prolongation of life span. The possibility that CoQ10 intake affects certain other biological functions by as yet unelucidated mechanisms cannot be ruled out as CoQ has been shown to broadly alter gene expression in mice.
辅酶Q（CoQ）在线粒体中具有三个已被充分表征的功能，即（i）在电子传递链中传递还原当量，（ii）生成超氧阴离子自由基O2•-，以及（iii）淬灭自由基。本综述的主要目的是在辅酶Q10给药是否能改变衰老过程整体进展这一更广泛问题的背景下，讨论相对长期摄入辅酶Q10对线粒体呼吸能力、氧化应激指标和动物寿命的影响。对不同哺乳动物物种的比较研究表明，线粒体超氧阴离子自由基的生成速率与线粒体辅酶Q9含量直接相关，与辅酶Q10的量呈负相关，特别是与结合在线粒体膜蛋白上的辅酶Q10。与历史观点相反，已证明给小鼠和大鼠补充辅酶Q10饮食可增加线粒体和各种组织匀浆中的内源性辅酶Q含量（辅酶Q9 + 辅酶Q10），尽管程度不同。摄入辅酶Q10会导致内源性辅酶Q9升高，这是小鼠和大鼠中的主要同系物。在我们的研究中，没有迹象表明摄入辅酶Q10会对线粒体呼吸活性、抗氧化能力、促氧化增强或寿命延长产生明显影响。由于已证明辅酶Q可广泛改变小鼠的基因表达，因此不能排除辅酶Q10摄入通过尚未阐明的机制影响某些其他生物学功能的可能性。
