Chieco P, Derenzini M
Istituto di Oncologia F. Addarii, Policlinico S. Orsola, Bologna, Italy.
Histochem Cell Biol. 1999 May;111(5):345-58. doi: 10.1007/s004180050367.
The Feulgen reaction proposed by Feulgen and Rossenbeck 75 years ago is one of the cytohistochemical reactions most widely used in biology and medicine. It allows DNA in situ to be specifically stained based on the reaction of Schiff or Schiff-like reagents with aldehyde groups engendered in the deoxyribose molecules by HCl hydrolysis. The staining intensity is proportional to the DNA concentration. Current applications of the Feulgen reaction are mainly concerned with DNA quantification in cell nuclei by image cytometry for ploidy evaluation in tumor pathology. From the morphological point of view, specific demonstration of DNA in cell structures at the light microscopic level is very little used nowadays. On the other hand, application of the Feulgen principles to electron microscopy have recently allowed specific DNA-staining procedures to be developed for the study of the structural organization of DNA in situ.
75年前由福尔根和罗斯森贝克提出的福尔根反应，是生物学和医学中应用最为广泛的细胞组织化学反应之一。基于席夫试剂或类席夫试剂与盐酸水解脱氧核糖分子产生的醛基的反应，该反应能够对原位DNA进行特异性染色。染色强度与DNA浓度成正比。福尔根反应目前的应用主要涉及通过图像细胞术对细胞核中的DNA进行定量分析，以用于肿瘤病理学中的倍性评估。从形态学角度来看，如今在光学显微镜水平对细胞结构中的DNA进行特异性显示的应用非常少。另一方面，将福尔根原理应用于电子显微镜，最近已开发出用于原位研究DNA结构组织的特异性DNA染色程序。
