Hadnagy W, Seemayer N H, Kühn K H, Leng G, Idel H
Institute of Hygiene, Heinrich-Heine University Düsseldorf, Germany.
Toxicol Lett. 1999 Jun 30;107(1-3):81-7. doi: 10.1016/s0378-4274(99)00034-x.
Five pyrethroids (fenvalerate, deltamethrin, cypermethrin, permethrin, cyfluthrin) differing in their chemical purity were investigated on their cytotoxic effects, especially on their ability to induce mitotic cell division disturbances using Chinese hamster lung cells of line V79. The colony forming ability (CFA) resulted in distinct differences of the cytotoxic effect of the tested pyrethroids, whereby permethrin was found to be most toxic. With the exception of fenvalerate all tested pyrethroids gave rise to inhibition of cell cycle progression as shown by G2/M-arrest of synchronized V79 cells by flow cytometry as well as by the increase of the mitotic index as evaluated by light microscopy. The mitotic arresting activity could be attributed to the occurrence of abnormal mitotic figures such as initial and full C-metaphases. The results however indicate, that pyrethroids per se do not contribute to the cytotoxic effects but that other factors such as chemical impurities, source as well as manufacturing process and isomer composition may be responsible for the observed cytotoxic effects.
研究了化学纯度不同的五种拟除虫菊酯（氰戊菊酯、溴氰菊酯、氯氰菊酯、氯菊酯、氟氯氰菊酯）对细胞的毒性作用，特别是利用V79系中国仓鼠肺细胞诱导有丝分裂细胞分裂紊乱的能力。集落形成能力（CFA）导致所测试的拟除虫菊酯的细胞毒性作用存在明显差异，其中氯菊酯被发现毒性最大。除氰戊菊酯外，所有测试的拟除虫菊酯均导致细胞周期进程受到抑制，这通过流式细胞术检测同步化的V79细胞的G2/M期阻滞以及通过光学显微镜评估有丝分裂指数的增加得以体现。有丝分裂阻滞活性可归因于异常有丝分裂图像的出现，如初期和完全C中期。然而，结果表明，拟除虫菊酯本身并不会导致细胞毒性作用，而是其他因素，如化学杂质、来源以及制造工艺和异构体组成，可能是观察到的细胞毒性作用的原因。
