Alonso J L, Soriano A, Carbajo O, Amoros I, Garelick H
Instituto de Hidrologia y Medio Natural, Universidad Politecnica, 46022 Valencia, Spain.
Appl Environ Microbiol. 1999 Aug;65(8):3746-9. doi: 10.1128/AEM.65.8.3746-3749.1999.
This study compared the performance of a commercial chromogenic medium, CHROMagarECC (CECC), and CECC supplemented with sodium pyruvate (CECCP) with the membrane filtration lauryl sulfate-based medium (mLSA) for enumeration of Escherichia coli and non-E. coli thermotolerant coliforms (KEC). To establish that we could recover the maximum KEC and E. coli population, we compared two incubation temperature regimens, 41 and 44.5 degrees C. Statistical analysis by the Fisher test of data did not demonstrate any statistically significant differences (P = 0.05) in the enumeration of E. coli for the different media (CECC and CECCP) and incubation temperatures. Variance analysis of data performed on KEC counts showed significant differences (P = 0.01) between KEC counts at 41 and 44.5 degrees C on both CECC and CECCP. Analysis of variance demonstrated statistically significant differences (P = 0.05) in the enumeration of total thermotolerant coliforms (TTCs) on CECC and CECCP compared with mLSA. Target colonies were confirmed to be E. coli at a rate of 91.5% and KEC of likely fecal origin at a rate of 77.4% when using CECCP incubated at 41 degrees C. The results of this study showed that CECCP agar incubated at 41 degrees C is efficient for the simultaneous enumeration of E. coli and KEC from river and marine waters.
本研究比较了一种商业显色培养基CHROMagarECC（CECC）以及添加丙酮酸钠的CECC（CECCP）与基于月桂基硫酸盐的膜过滤培养基（mLSA）对大肠杆菌和非大肠杆菌耐热大肠菌群（KEC）进行计数的性能。为确定我们能够回收最大数量的KEC和大肠杆菌菌群，我们比较了41℃和44.5℃两种培养温度方案。通过Fisher检验对数据进行的统计分析表明，对于不同培养基（CECC和CECCP）及培养温度，大肠杆菌计数没有任何统计学上的显著差异（P = 0.05）。对KEC计数进行的数据方差分析显示，在CECC和CECCP上，41℃和44.5℃时的KEC计数存在显著差异（P = 0.01）。方差分析表明，与mLSA相比，CECC和CECCP上总耐热大肠菌群（TTC）计数存在统计学上的显著差异（P = 0.05）。当使用在41℃培养的CECCP时，目标菌落被确认为大肠杆菌的比例为91.5%，可能源自粪便的KEC比例为77.4%。本研究结果表明，在41℃培养的CECCP琼脂对同时计数河流和海水中的大肠杆菌和KEC是有效的。
