Ciebin B W, Brodsky M H, Eddington R, Horsnell G, Choney A, Palmateer G, Ley A, Joshi R, Shears G
Ontario Ministry of Health, Laboratory Services Branch, Toronto, Canada.
Appl Environ Microbiol. 1995 Nov;61(11):3940-2. doi: 10.1128/aem.61.11.3940-3942.1995.
Two media used to detect fecal coliforms in water by membrane filtration, m-FC and m-TEC, were modified and supplemented with the chromogenic substrate 5-bromo-6-chloro-3-indoyl-beta-D-glucuronide (BCIG) and were compared for quantitative recovery of Escherichia coli. Student's t test of data from 181 water samples of sewage, rivers, lakes, and wells did not demonstrate any statistically significant differences (P = 0.05) in the enumeration of E. coli with these media. Target colonies were confirmed to be E. coli at rates of 98.6 and 97.3% by using FC-BCIG and TEC-BCIG media, respectively. Glucuronidase-negative isolates of E. coli were encountered at the same frequency (6.0%) on both media. This collaborative study demonstrated that either modified basal medium could be used successfully for detection of E. coli in various nontreated waters within 24 h.
用于通过膜过滤法检测水中粪大肠菌群的两种培养基，即m-FC和m-TEC，经改良后添加了显色底物5-溴-6-氯-3-吲哚-β-D-葡萄糖醛酸苷（BCIG），并对其进行了比较，以定量回收大肠杆菌。对来自污水、河流、湖泊和水井的181个水样的数据进行的学生t检验表明，使用这些培养基对大肠杆菌进行计数时，没有显示出任何统计学上的显著差异（P = 0.05）。使用FC-BCIG和TEC-BCIG培养基时，目标菌落被确认为大肠杆菌的比率分别为98.6%和97.3%。在两种培养基上，大肠杆菌的葡萄糖醛酸酶阴性分离株出现的频率相同（6.0%）。这项合作研究表明，两种改良的基础培养基均可在24小时内成功用于检测各种未处理水中的大肠杆菌。
