Cacciatore T W, Brodfuehrer P D, Gonzalez J E, Jiang T, Adams S R, Tsien R Y, Kristan W B, Kleinfeld D
Group in Neurosciences, University of California, San Diego, La Jolla 92093, USA.
Neuron. 1999 Jul;23(3):449-59. doi: 10.1016/s0896-6273(00)80799-0.
We show that neurons that underlie rhythmic patterns of electrical output may be identified by optical imaging and frequency-domain analysis. Our contrast agent is a two-component dye system in which changes in membrane potential modulate the relative emission between a pair of fluorophores. We demonstrate our methods with the circuit responsible for fictive swimming in the isolated leech nerve cord. The output of a motor neuron provides a reference signal for the phase-sensitive detection of changes in fluorescence from individual neurons in a ganglion. We identify known and possibly novel neurons that participate in the swim rhythm and determine their phases within a cycle. A variant of this approach is used to identify the postsynaptic followers of intracellularly stimulated neurons.
我们表明，可通过光学成像和频域分析来识别构成电输出节律模式的神经元。我们的造影剂是一种双组分染料系统，其中膜电位的变化会调节一对荧光团之间的相对发射。我们用负责离体水蛭神经索中虚构游泳的回路来演示我们的方法。运动神经元的输出为对神经节中单个神经元荧光变化进行相敏检测提供参考信号。我们识别出参与游泳节律的已知和可能的新神经元，并确定它们在一个周期内的相位。这种方法的一个变体用于识别细胞内刺激神经元的突触后跟随者。
