Freymuth F, Vabret A, Brouard J, Toutain F, Verdon R, Petitjean J, Gouarin S, Duhamel J F, Guillois B
Laboratory of Human and Molecular Virology, University Hospital, Caen, France.
J Clin Virol. 1999 Aug;13(3):131-9. doi: 10.1016/s1386-6532(99)00030-x.
A high frequency of virus infections has been recently pointed out in the exacerbations of asthma in children.
To confirm this, using conventional and molecular detection methods, and expanding the study to younger children.
One hundred and thirty-two nasal aspirates from 75 children hospitalized for a severe attack of asthma were studied (32 infants, mean age 9.1 months; and 43 children, mean age 5.6 years). According to the virus, a viral isolation technique, immunofluorescence assays (IFA) or both were used for the detection of rhinovirus, enterovirus, respiratory syncytial (RS) virus, adenovirus, coronavirus 229E, influenza and parainfluenza virus. Polymerase chain reaction (PCR) assays were used for the detection of rhinovirus, enterovirus, RS virus, adenovirus, coronavirus 229E and OC43, Chlamydia pneumoniae and Mycoplasma pneumoniae.
Using IFA and viral isolation techniques, viruses were detected in 33.3% of cases, and by PCR techniques, nucleic acid sequences of virus, Chlamydia pneumoniae and Mycoplasma pneumoniae were obtained in 71.9% of cases. The combination of conventional and molecular techniques detects 81.8% of positive samples. Two organisms were identified in the same nasal sample in 20.4% of the cases. The percentage of detections was higher (85.9%) in the younger group than in the other (77%). The most frequently detected agents were rhinovirus (46.9%) and RS virus (21.2%). Using PCR rather than conventional techniques, the detection rates were increased 5.8- and 1.6-fold in rhinovirus and RS virus infections, respectively. The detection levels of the other organisms are as follows: 9.8, 5.1, 4.5, 4.5, 4.5, 3.7, and 2.2% for enterovirus, influenza virus, Chlamydia pneumoniae, adenovirus, coronavirus, parainfluenza virus, and Mycoplasma pneumoniae, respectively.
These results confirm the previously reported high frequency of rhinovirus detection in asthmatic exacerbations in children. They also point out the frequency of RS virus detection, and emphasize the fact that PCR assays may be necessary to diagnose respiratory infections in asthma.
最近有研究指出，儿童哮喘急性发作时病毒感染的频率较高。
采用传统和分子检测方法对此进行确认，并将研究范围扩大到更小的儿童。
对75名因哮喘严重发作住院的儿童的132份鼻吸出物进行了研究（32名婴儿，平均年龄9.1个月；43名儿童，平均年龄5.6岁）。根据病毒类型，采用病毒分离技术、免疫荧光测定法（IFA）或两者结合来检测鼻病毒、肠道病毒、呼吸道合胞（RS）病毒、腺病毒、冠状病毒229E、流感病毒和副流感病毒。聚合酶链反应（PCR）测定法用于检测鼻病毒、肠道病毒、RS病毒、腺病毒、冠状病毒229E和OC43、肺炎衣原体和肺炎支原体。
采用IFA和病毒分离技术，在33.3%的病例中检测到病毒；通过PCR技术，在71.9%的病例中获得了病毒、肺炎衣原体和肺炎支原体的核酸序列。传统技术与分子技术相结合可检测出81.8%的阳性样本。在20.4%的病例中，同一份鼻样本中鉴定出两种病原体。年龄较小的组检测率较高（85.9%），高于另一组（77%）。最常检测到的病原体是鼻病毒（46.9%）和RS病毒（21.2%）。与传统技术相比，采用PCR技术时，鼻病毒和RS病毒感染的检测率分别提高了5.8倍和1.6倍。其他病原体的检测水平如下：肠道病毒、流感病毒、肺炎衣原体、腺病毒、冠状病毒、副流感病毒和肺炎支原体分别为9.8%、5.1%、4.5%、4.5%、4.5%、3.7%和2.2%。
这些结果证实了先前报道的儿童哮喘急性发作时鼻病毒检测频率较高。它们还指出了RS病毒的检测频率，并强调在诊断哮喘患者的呼吸道感染时可能需要采用PCR测定法。
