Zobeley E, Flechsig E, Cozzio A, Enari M, Weissmann C
Institute of Molecular Biology, University of Zürich, Switzerland.
Mol Med. 1999 Apr;5(4):240-3.
The transmissible agent of Creutzfeldt-Jakob disease (CJD) is not readily destroyed by conventional sterilization and transmissions by surgical instruments have been reported. Decontamination studies have been carried out thus far on solutions or suspensions of the agent and may not reflect the behavior of surface-bound infectivity.
As a model for contaminated surgical instruments, thin stainless-steel wire segments were exposed to scrapie agent, washed exhaustively with or without treatment with 10% formaldehyde, and implanted into the brains of indicator mice. Infectivity was estimated from the time elapsing to terminal disease.
Stainless steel wire (0.15 x 5 mm) exposed to scrapie-infected mouse brain homogenate and washed extensively with PBS retained the equivalent of about 10(5) LD50 units per segment. Treatment with 10% formaldehyde for 1 hr reduced this value by only about 30-fold.
The model system we have devised confirms the anecdotal reports that steel instruments can retain CJD infectivity even after formaldehyde treatment. It lends itself to a systematic study of the conditions required to effectively inactivate CJD, bovine spongiform encephalopathy, and scrapie agent adsorbed to stainless steel surfaces such as those of surgical instruments.
克雅氏病（CJD）的传播因子不易被常规消毒方法破坏，且已有手术器械传播该病的报道。迄今为止，针对该传播因子的去污研究是在其溶液或悬浮液上进行的，可能无法反映表面结合传染性的行为。
作为受污染手术器械的模型，将细不锈钢丝段暴露于羊瘙痒病因子中，在有或没有用10%甲醛处理的情况下进行彻底清洗，然后植入指示小鼠的大脑。根据直至终末期疾病的时间来估计传染性。
暴露于羊瘙痒病感染小鼠脑匀浆并经PBS广泛清洗的不锈钢丝（0.15×5mm），每段保留约10⁵半数致死剂量（LD50）单位的等效量。用10%甲醛处理1小时仅使该值降低约30倍。
我们设计的模型系统证实了轶事报道，即即使经过甲醛处理，钢制器械仍可保留CJD传染性。它有助于系统研究有效灭活吸附在不锈钢表面（如手术器械表面）的CJD、牛海绵状脑病和羊瘙痒病因子所需的条件。
