免疫球蛋白M抗体检测以检测基因组II型诺如病毒感染。

Immunoglobulin M antibody test to detect genogroup II Norwalk-like virus infection.

作者信息

Brinker J P, Blacklow N R, Jiang X, Estes M K, Moe C L, Herrmann J E

机构信息

Division of Infectious Diseases and Immunology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA.

出版信息

J Clin Microbiol. 1999 Sep;37(9):2983-6. doi: 10.1128/JCM.37.9.2983-2986.1999.

DOI:10.1128/JCM.37.9.2983-2986.1999

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC85428/

Abstract

Sera obtained from adult volunteers inoculated with genogroup II Norwalk-like viruses (NLVs), Hawaii virus, and Snow Mountain virus and from patients involved in outbreaks of gastroenteritis were tested for genogroup II NLV Mexico virus-specific immunoglobulin M (IgM) by use of a monoclonal antibody, recombinant Mexico virus antigen (rMXV)-based IgM capture enzyme-linked immunosorbent assay (ELISA). Sera from genogroup I Norwalk virus (NV)-inoculated volunteers and from patients involved in a genogroup I NLV outbreak were also tested. In sera from those infected with genogroup I NV or NLVs in volunteer and outbreak studies, only 3 of 25 were rMXV IgM positive; in contrast, 24 of 25 were IgM positive for recombinant NV (rNV). In sera from those infected with genogroup II NLVs in volunteer and outbreak studies, 28 of 47 were rMXV IgM positive and none were IgM positive for rNV, showing the specificity of each IgM test for its respective genogroup. In an outbreak of gastroenteritis not characterized as being of viral etiology but suspected to be due to NV, 7 of 13 persons had IgM responses to rMXV, whereas none had IgM responses to rNV, thus establishing the diagnosis as genogroup II NLV infection. The rMXV-based IgM capture ELISA developed is specific for the diagnosis of genogroup II NLV infections.

摘要

从接种了基因群II诺如病毒样病毒（NLVs）、夏威夷病毒和雪山病毒的成年志愿者以及参与肠胃炎暴发的患者中获得的血清，采用基于单克隆抗体、重组墨西哥病毒抗原（rMXV）的IgM捕获酶联免疫吸附测定（ELISA），检测其针对基因群II NLV墨西哥病毒的特异性免疫球蛋白M（IgM）。还检测了接种基因群I诺如病毒（NV）的志愿者以及参与基因群I NLV暴发的患者的血清。在志愿者和暴发研究中感染基因群I NV或NLVs的个体血清中，25份血清中只有3份rMXV IgM呈阳性；相比之下，25份血清中有24份重组NV（rNV）IgM呈阳性。在志愿者和暴发研究中感染基因群II NLVs的个体血清中，47份血清中有28份rMXV IgM呈阳性，且无一份rNV IgM呈阳性，这表明每种IgM检测方法对其相应基因群具有特异性。在一次未被确认为病毒病因但怀疑由NV引起的肠胃炎暴发中，13人中有7人对rMXV有IgM反应，而无人对rNV有IgM反应，从而确诊为基因群II NLV感染。所开发的基于rMXV的IgM捕获ELISA对基因群II NLV感染的诊断具有特异性。