Both stump area and volume of distal sensory nerve segments influence the regeneration of sensory axons in rats.

We examined the influence of both stump area and volume of a distal sensory nerve segment on neurotropic induction of regenerating sensory axons in a rat saphenous nerve model. In group 1 (n = 10) the proximal stump of the severed saphenous nerve was inserted into the proximal channel, and a 2 cm free nerve segment and a double-barrelled 1 cm free nerve segment were inserted into the distal two channels of a silicone Y-chamber. In group 2 (n = 10), 2 cm and 1 cm free nerve segments were inserted into the distal two channels of a Y-chamber. The gap between the stumps was set at 4 mm. After six weeks, we counted and compared the number of regenerated myelinated sensory axons in the distal two channels. Significantly more axons regenerated in the wider stump area channel of group 1 and in the larger volume channel of group 2 than in the opposite channel in either group (p < 0.05 in each case).