Huang G T, Potente A P, Kim J W, Chugal N, Zhang X
Section of Oral Biology and Dental Research Institute, UCLA School of Dentistry, Los Angeles, CA 90095-1668. USA.
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 1999 Aug;88(2):214-20. doi: 10.1016/s1079-2104(99)70118-6.
Elevated levels of interleukin-8, a potent chemoattractant and activator of neutrophils, are associated with infectious and inflammatory diseases. However, little is known about interleukin-8 expression in human dental pulp. The purpose of this study was to determine whether tissue levels of interleukin-8 are elevated in irreversibly inflamed human pulps.
Experimental samples were from teeth clinically diagnosed with irreversible pulpitis (diseased pulps). Controls were from freshly extracted, caries-free third molars (normal pulps). Samples were subjected to enzyme-linked immunosorbent assay and/or immunohistochemical analysis with specific antibodies to interleukin-8.
The enzyme-linked immunosorbent assay studies showed elevated levels of interleukin-8 in diseased pulps (mean, 1.82+/-0.79 pg/mL/microg protein), as compared to detectable interleukin-8 levels in samples from normal pulps (mean, 0.08+/-0.04 pg/mL/microg protein; P<.05). Immunohistochemical analyses demonstrated that diseased samples exhibited a higher density of localized interleukin-8 staining in areas with heavy infiltration of inflammatory cells. In contrast, normal pulps showed negative or weak interleukin-8 staining.
Interleukin-8 concentration was higher in pulps diagnosed with irreversible pulpitis; only negligible amounts of interleukin-8 were present in normal pulps.
白细胞介素-8是一种强效的中性粒细胞趋化因子和激活剂，其水平升高与感染性和炎症性疾病相关。然而，关于白细胞介素-8在人牙髓中的表达情况却知之甚少。本研究的目的是确定在不可逆性炎症牙髓中白细胞介素-8的组织水平是否升高。
实验样本来自临床诊断为不可逆性牙髓炎的牙齿（患病牙髓）。对照组来自新鲜拔除的、无龋的第三磨牙（正常牙髓）。样本采用酶联免疫吸附测定法和/或用针对白细胞介素-8的特异性抗体进行免疫组织化学分析。
酶联免疫吸附测定研究显示，患病牙髓中白细胞介素-8水平升高（平均为1.82±0.79 pg/mL/μg蛋白质），而正常牙髓样本中白细胞介素-8水平可检测到（平均为0.08±0.04 pg/mL/μg蛋白质；P<0.05）。免疫组织化学分析表明，患病样本在炎症细胞大量浸润区域呈现出更高密度的局部白细胞介素-8染色。相比之下，正常牙髓显示白细胞介素-8染色阴性或弱阳性。
诊断为不可逆性牙髓炎的牙髓中白细胞介素-8浓度更高；正常牙髓中仅存在可忽略不计的白细胞介素-8量。
