Jiang Hong-Wei, Ling Jun-Qi, Gong Qi-Mei
Department of Operative and Endodontics, Guanghua College of Stomatology, Sun Yat-sen University, Guangzhou, China.
Department of Operative and Endodontics, Guanghua College of Stomatology, Sun Yat-sen University, Guangzhou, China.
J Endod. 2008 Nov;34(11):1351-1354. doi: 10.1016/j.joen.2008.07.023. Epub 2008 Aug 30.
Although dental pulp progenitor/stem cells (DPSCs) are indispensable for repair after pulpal injury, the mechanisms regulating their recruitment and activation remain unknown. To address this issue, we evaluated whether DPSCs in inflamed dental pulp had an upregulation of the chemokine system, a system of proteins known to regulate cellular responses to inflammation. Stromal cell-derived factor 1 (SDF-1), a member of the CXC chemokine subfamily and its receptor CXC chemokine receptor 4 (CXCR4), were evaluated in inflamed dental pulps obtained from extracted human teeth that showed spontaneous pain and/or lingering pain in response to cold and/or heat stimulus and compared with control levels found in normal dental pulps obtained from healthy noncarious third molars. Using immunohistochemistry and real-time reverse-transcription polymerase chain reaction, the results indicated that in inflamed pulps the SDF-1/CXCR4 axis was mostly distributed in inflammatory cells and microvascular endothelial cells rather than in normal pulps. SDF-1 messenger RNA expression levels in clinically inflamed dental pulp were higher than those in healthy dental pulp. These findings suggest that SDF-1 plays an important role in the process of pulpal inflammation via the recruitment of CXCR4-expressing inflammatory cells, and the SDF-1/CXCR4 axis may be involved in the recruitment of dental pulp stem cells at the injury site.
尽管牙髓祖细胞/干细胞（DPSCs）在牙髓损伤后的修复过程中不可或缺，但其募集和激活的调控机制仍不清楚。为了解决这个问题，我们评估了炎症牙髓中的DPSCs是否上调了趋化因子系统，这是一个已知可调节细胞对炎症反应的蛋白质系统。在从表现出自发性疼痛和/或对冷和/或热刺激有持续性疼痛的拔除人类牙齿中获取的炎症牙髓中，评估了基质细胞衍生因子1（SDF-1），它是CXC趋化因子亚家族的成员及其受体CXC趋化因子受体4（CXCR4），并与从健康无龋第三磨牙获取的正常牙髓中的对照水平进行比较。使用免疫组织化学和实时逆转录聚合酶链反应，结果表明，在炎症牙髓中，SDF-1/CXCR4轴主要分布在炎症细胞和微血管内皮细胞中，而不是在正常牙髓中。临床炎症牙髓中SDF-1信使核糖核酸表达水平高于健康牙髓。这些发现表明，SDF-1通过募集表达CXCR4的炎症细胞在牙髓炎症过程中发挥重要作用，并且SDF-1/CXCR4轴可能参与损伤部位牙髓干细胞的募集。
