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用于在硝酸纤维素纸上检测抗原的免疫金法。

Immunogold for detection of antigen on nitrocellulose paper.

作者信息

Hsu Y H

出版信息

Anal Biochem. 1984 Oct;142(1):221-5. doi: 10.1016/0003-2697(84)90542-6.

DOI:10.1016/0003-2697(84)90542-6
PMID:6083735
Abstract

The application of immunogold for the detection of antigen-antibody complexes on nitrocellulose paper is described. Tobacco mosaic virus (TMV) protein, either purified or in leaf extract, was bound to the nitrocellulose paper and then exposed to rabbit anti-TMV serum. The antigen-antibody complexes were detected by gold-labeled goat anti-rabbit immunoglobulin G. The gold-labeled antibody is directly visible because of its pink color. This method can detect 1-5 pg of TMV protein, either in purified form or in the unpurified plant extract, with high specificity.

摘要

本文描述了免疫金在硝酸纤维素纸上检测抗原-抗体复合物的应用。纯化的烟草花叶病毒（TMV）蛋白或叶提取物中的TMV蛋白与硝酸纤维素纸结合，然后与兔抗TMV血清接触。通过金标记的山羊抗兔免疫球蛋白G检测抗原-抗体复合物。由于金标记抗体呈粉红色，所以可直接观察到。该方法能以高特异性检测1-5皮克纯化形式或未纯化植物提取物中的TMV蛋白。

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