Bratanich A, Lairmore M, Heneine W, Konoby C, Harding J, West K, Vasquez G, Allan G, Ellis J
Department of Veterinary Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon.
Can J Vet Res. 1999 Jul;63(3):207-11.
In order to investigate the role of retroviruses in the recently described porcine postweaning multisystemic wasting syndrome (PMWS) serum and leukocytes were screened for reverse transcriptase (RT) activity, and tissues were examined for the presence of conserved lentiviral sequences using degenerate primers in a polymerase chain reaction (PCR). Serum and stimulated leukocytes from the blood and lymph nodes from pigs with PMWS, as well as from control pigs had RT activity that was detected by the sensitive Amp-RT assay. A 257-bp fragment was amplified from DNA from the blood and bone marrow of pigs with PMWS. This fragment was identical in size to conserved lentiviral sequences that were amplified from plasmids containing DNA from several lentiviruses. Cloning and sequencing of the fragment from affected pigs, however, did not reveal homology with the recognized lentiviruses. Together the results of these analyses suggest that the RT activity present in tissues from control and affected pigs is the result of endogenous retrovirus expression, and that a lentivirus is not a primary pathogen in PMWS.
为了研究逆转录病毒在最近描述的猪断奶后多系统消耗综合征（PMWS）中的作用，对血清和白细胞进行了逆转录酶（RT）活性筛查，并使用简并引物通过聚合酶链反应（PCR）检测组织中是否存在保守的慢病毒序列。患有PMWS的猪以及对照猪的血清、血液和淋巴结中经刺激的白细胞具有RT活性，可通过灵敏的Amp-RT检测法检测到。从患有PMWS的猪的血液和骨髓DNA中扩增出一个257 bp的片段。该片段的大小与从含有几种慢病毒DNA的质粒中扩增出的保守慢病毒序列相同。然而，对患病猪的片段进行克隆和测序后，未发现与已知慢病毒具有同源性。这些分析结果共同表明，对照猪和患病猪组织中存在的RT活性是内源性逆转录病毒表达的结果，并且慢病毒不是PMWS的主要病原体。
