Zirnstein G, Li Y, Swaminathan B, Angulo F
National Center for Infectious Diseases, Centers for Disease Control and Prevention, Public Health Service, U.S. Department of Health and Human Services, Atlanta, Georgia 30333, USA.
J Clin Microbiol. 1999 Oct;37(10):3276-80. doi: 10.1128/JCM.37.10.3276-3280.1999.
The gyrA quinolone resistance determining region was sequenced from 13 ciprofloxacin-resistant and 20 ciprofloxacin-susceptible Campylobacter jejuni isolates. All isolates resistant to ciprofloxacin had Thr-86-to-Ile mutations, a mutation frequently associated with the acquisition of resistance to fluoroquinolones. A mismatch amplification mutation assay (MAMA) PCR protocol was developed that detects this gyrA mutation in quinolone-resistant isolates. The MAMA PCR provides a means for routine detection of the gyrA mutation without the need for sequencing the gyrA gene.
对13株耐环丙沙星和20株对环丙沙星敏感的空肠弯曲菌分离株的gyrA喹诺酮耐药决定区进行了测序。所有耐环丙沙星的分离株均有86位苏氨酸到异亮氨酸的突变，该突变常与对氟喹诺酮类药物耐药性的获得有关。开发了一种错配扩增突变分析（MAMA）PCR方案，可检测喹诺酮耐药分离株中的这种gyrA突变。MAMA PCR提供了一种无需对gyrA基因进行测序即可常规检测gyrA突变的方法。
