Li Fang, Lv Chongning, Li Qiao, Wang Jing, Song Dan, Liu Pengpeng, Zhang Dandan, Lu Jincai
School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang, China.
Department of Pharmacy, Affiliated Hospital, Liaoning University of Traditional Chinese Medicine, Shenyang, China.
J Ginseng Res. 2017 Oct;41(4):487-495. doi: 10.1016/j.jgr.2016.08.008. Epub 2016 Aug 26.
Although flowers of Meyer (FPG), L. (FPQ), and Burk. (FPN) have been historically used as both medicine and food, each is used differently in practice.
To investigate the connection between components and enhancing immunity activity of FPG, FPQ, and FPN, a method based on a rapid LC coupled with quadrupole time-of-flight MS and immunomodulatory activity study evaluated by a carbon clearance test were combined.
According to quantitative results, the ratio of the total content of protopanaxatiol-type ginsenosides to protopanaxadiol-type ginsenosides in FPN was 0, but ranged from 1.10 to 1.32 and from 0.23 to 0.35 in FPG and FPQ, respectively. The ratio of the total content of neutral ginsenosides to the corresponding malonyl-ginsenosides in FPN (5.52 ± 1.33%) was higher than FPG (3.2 ± 0.64%) and FPQ (2.39 ± 0.57%). The colorimetric analysis showed the content of total ginsenosides in FPQ, FPG, and FPN to be 13.75 ± 0.60%, 17.45 ± 0.42%, and 12.45 ± 1.77%, respectively. The carbon clearance assay indicated that the phagocytic activity of FPG and FPQ was higher than that of FPN. A clear discrimination among FPG, FPQ, and FPN was observed in the principal component analysis score plots. Seven compounds were confirmed to contribute strongly by loading plots, which may be the cause of differences in efficacy.
This study provides basic information about the chemical and bioactive comparison of FPG, FPQ, and FPN, indicating that protopanaxtriol-type ginsenosides and malonyl-ginsenosides may play a key role in their enhancing immunity properties.
虽然迈耶氏（FPG）、L.（FPQ）和伯克氏（FPN）的花在历史上一直被用作药物和食物，但在实际应用中它们的用途各不相同。
为了研究FPG、FPQ和FPN的成分与增强免疫活性之间的联系，将基于快速液相色谱联用四极杆飞行时间质谱的方法与通过碳清除试验评估的免疫调节活性研究相结合。
根据定量结果，FPN中原人参三醇型人参皂苷与原人参二醇型人参皂苷的总含量之比为0，而FPG和FPQ中该比例分别为1.10至1.32以及0.23至0.35。FPN中中性人参皂苷与相应丙二酰人参皂苷的总含量之比（5.52±1.33%）高于FPG（3.2±0.64%）和FPQ（2.39±0.57%）。比色分析表明，FPQ、FPG和FPN中总人参皂苷的含量分别为13.75±0.60%、17.45±0.42%和12.45±1.77%。碳清除试验表明，FPG和FPQ的吞噬活性高于FPN。在主成分分析得分图中观察到FPG、FPQ和FPN之间有明显区分。通过载荷图确认有七种化合物起了重要作用，这可能是功效差异的原因。
本研究提供了关于FPG、FPQ和FPN的化学和生物活性比较的基本信息，表明原人参三醇型人参皂苷和丙二酰人参皂苷可能在其增强免疫特性中起关键作用。
