Klieve A V, Heck G L, Prance M A, Shu Q
Queensland Beef Industry Institute, Queensland Department of Primary Industries, Moorooka, Australia.
Lett Appl Microbiol. 1999 Aug;29(2):108-12. doi: 10.1046/j.1365-2672.1999.00596.x.
The genetic homogeneity of 37 strains of ruminal streptococci was investigated by comparing DNA fragment profiles on agarose gels following restriction endonuclease digestion with Hae III, Cfo I and Msp I. Thirty strains were indistinguishable from Streptococcus bovis strains, 2B, H24 and AR3. The remaining three strains were similar but not identical to a ruminal strain of Strep. intermedius (AR36). In addition, the susceptibility of these strains to infection by five bacteriophages was examined. Three of the phages (phi Sb02, phi Sb03 and phi Sb04) were specific to the strain of Strep. bovis from which they were isolated, while phages 2BV and phi Sb01 infected one and two strains, respectively, in addition to their primary host. It was concluded that although Strep. bovis is relatively homogeneous genetically, broad host range phages appear to be uncommon with this bacterial species.
通过比较用Hae III、Cfo I和Msp I限制性内切酶消化后在琼脂糖凝胶上的DNA片段图谱，研究了37株瘤胃链球菌的遗传同质性。30株与牛链球菌菌株2B、H24和AR3无法区分。其余三株与中间链球菌的瘤胃菌株（AR36）相似但不完全相同。此外，还检测了这些菌株对五种噬菌体感染的敏感性。其中三种噬菌体（phi Sb02、phi Sb03和phi Sb04）对分离出它们的牛链球菌菌株具有特异性，而噬菌体2BV和phi Sb01除了感染其原始宿主外，还分别感染了一株和两株其他菌株。得出的结论是，尽管牛链球菌在遗传上相对同质，但该细菌物种中广泛宿主范围的噬菌体似乎并不常见。
