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使用酸性电解水进行内镜消毒。

Endoscope disinfection using acidic electrolytic water.

作者信息

Tsuji S, Kawano S, Oshita M, Ohmae A, Shinomura Y, Miyazaki Y, Hiraoka S, Matsuzawa Y, Kamada T, Hori M, Maeda T

机构信息

First Dept. of Medicine, Osaka University School of Medicine, Suita, Japan.

出版信息

Endoscopy. 1999 Sep;31(7):528-35. doi: 10.1055/s-1999-55.

Abstract

BACKGROUND AND STUDY AIMS

The aim of the present study was to evaluate a new endoscope disinfector (WM-1) that uses acidic electrolytic water (AEW).

MATERIALS AND METHODS

AEW was produced by electrolysis of a 0.05% NaCl-water mixture, with a redox potential greater than 1000 mV and a pH lower than 2.7. In the first study, an endoscope artificially contaminated with 15 species of bacteria and four strains of viruses was treated using the WM-1. In the second study, endoscopic contamination after clinical use was examined by culture for Helicobacter pylori and other bacteria, and by polymerase chain reaction for the H. pylori urease gene and hepatitis C virus. The extent of contamination was then examined after exposing the WM-1 to AEW. The safety of AEW was examined using both in vivo and in vitro studies.

RESULTS

All of the bacteria and viruses were destroyed or inactivated after the instrument had been exposed to AEW. Clinical contamination was detected from the instrument in 19 of 30 endoscopic procedures, whereas no bacteria or viruses were detected after five minutes' exposure to AEW. AEW was found to be nonirritant, nontoxic to cells, and nonmutagenic.

CONCLUSION

The WM-1 successfully and safely disinfected the endoscopes. With running costs of yen 24 per day ($0.21 per day), the WM-1 provides an effective and inexpensive alternative to conventional disinfection equipment.

摘要

背景与研究目的

本研究旨在评估一种使用酸性电解水(AEW)的新型内镜消毒器(WM - 1)。

材料与方法

通过电解0.05%的氯化钠 - 水混合物产生AEW,其氧化还原电位大于1000 mV,pH值低于2.7。在第一项研究中,使用WM - 1对人工沾染15种细菌和4种病毒株的内镜进行处理。在第二项研究中,通过对幽门螺杆菌和其他细菌进行培养,以及对幽门螺杆菌脲酶基因和丙型肝炎病毒进行聚合酶链反应,来检测临床使用后内镜的污染情况。然后在将WM - 1暴露于AEW后检查污染程度。使用体内和体外研究来检查AEW的安全性。

结果

在器械暴露于AEW后,所有细菌和病毒均被破坏或灭活。在30例内镜检查操作中的19例中,从器械上检测到临床污染,而在暴露于AEW五分钟后未检测到细菌或病毒。发现AEW无刺激性,对细胞无毒,且无致突变性。

结论

WM - 1成功且安全地对内窥镜进行了消毒。WM - 1每天的运行成本为24日元(0.21美元/天),为传统消毒设备提供了一种有效且廉价的替代方案。

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