Matsuo N
Department of Emergency, Kansai Medical University, 10-15 Fumizono-cho, Moriguchi, Osaka 570-8507, Japan.
Surg Today. 1999;29(10):1068-74. doi: 10.1007/s005950050646.
This study was conducted to investigate the role of nitric oxide (NO) in the development of adult respiratory distress syndrome (ARDS). An experimental model of endotoxemia-induced ARDS was prepared in guinea pigs and the following parameters were measured: pulmonary vascular permeability, the nitrate and nitrite concentrations in blood and bronchoalveolar lavage fluid (BALF), and the activities of constitutive and inducible NO synthase in lung tissue following the administration of lipopolysaccharide (LPS). Following the intravenous administration of 0.5 mg/kg LPS, the pulmonary vascular permeability increased, as did the concentrations of nitrate and nitrite in the BALF and blood. The activities of both constitutive and inducible NO synthase (NOS) increased significantly in the lung tissue 4 h after the intravenous administration of LPS, the constitutive form showing significantly higher activity than the inducible form. Furthermore, the increase of vascular permeability in the lungs after LPS injection was blocked by the subcutaneous administration of N(G)-monomethyl-l-arginine. These observations indicate that the intrapulmonary generation of NO may play an important role in the development of ARDS.
本研究旨在探讨一氧化氮（NO）在成人呼吸窘迫综合征（ARDS）发生发展中的作用。在豚鼠中制备了内毒素血症诱导的ARDS实验模型，并测量了以下参数：肺血管通透性、血液和支气管肺泡灌洗（BALF）中的硝酸盐和亚硝酸盐浓度，以及脂多糖（LPS）给药后肺组织中组成型和诱导型NO合酶的活性。静脉注射0.5mg/kg LPS后，肺血管通透性增加，BALF和血液中的硝酸盐和亚硝酸盐浓度也增加。静脉注射LPS后4小时，肺组织中组成型和诱导型NO合酶（NOS）的活性均显著增加，组成型形式的活性显著高于诱导型形式。此外，皮下注射N（G）-单甲基-L-精氨酸可阻断LPS注射后肺血管通透性的增加。这些观察结果表明，肺内NO的产生可能在ARDS的发生发展中起重要作用。
