长春花冠瘿瘤组织中的细胞分裂因子。
Cell-division factors from Vinca rosca L. crown gall tumor tissue.
作者信息
Miller C O
出版信息
Proc Natl Acad Sci U S A. 1975 May;72(5):1883-6. doi: 10.1073/pnas.72.5.1883.
A cell-division factor has been precipitated from extracts of cultured Vinca rosea L. crown gall tumor tissue by using the mercuric acetate procedure previously employed by Wood and colleagues to obtain their "cytokinesin I." On the basis of its mass spectrum, ultraviolet light absorbancy spectra, solubilities, chromatographic migration values, and growth activity, the factor is ribosyl-trans-zeatin, that is, 6-(4-hydroxy-3-methyl-trans-2-butenylamino)-9-beta-D-ribofuranosylpurine. Ribosylzeatin has now been isolated from tumor tissue by four experimental techniques; any possibility that it is an artifact seems to have been eliminated. Contrary to the report by Wood and colleagues, synthetic ribosylzeatin is precipitated from an aqueous solution by mercuric acetate, provided the complete precipitation procedure is utilized. These facts and others discussed strongly support our suggestion that ribosylzeatin was present in the preparation ("cytokinesin I") examined by Wood and colleagues in several biological assays. The reasons advanced by Wood and others for rejecting this suggestion have been found either not to be pertinent to the question or to have insufficient experimental bases.
通过使用伍德及其同事之前用于获取其“细胞分裂素 I”的醋酸汞法，从培养的长春花冠瘿瘤组织提取物中沉淀出一种细胞分裂因子。根据其质谱、紫外光吸收光谱、溶解度、色谱迁移值和生长活性，该因子为核糖基反式玉米素，即6-(4-羟基-3-甲基反式-2-丁烯基氨基)-9-β-D-呋喃核糖基嘌呤。现已通过四种实验技术从肿瘤组织中分离出核糖基玉米素；它是人为产物的任何可能性似乎都已被排除。与伍德及其同事的报告相反，只要采用完整的沉淀程序，合成的核糖基玉米素就能从水溶液中被醋酸汞沉淀出来。这些事实以及所讨论的其他内容有力地支持了我们的观点，即核糖基玉米素存在于伍德及其同事在几种生物学测定中所检测的制剂（“细胞分裂素 I”）中。伍德等人拒绝这一观点所提出的理由已被发现要么与该问题无关，要么缺乏足够的实验依据。
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