Steven F S, Katsumi T, Payne P W, Denton J
Division of Biochemistry, School of Biological Sciences, University of Manchester, U.K.
Anticancer Res. 1999 Jul-Aug;19(4C):3491-3.
Sputum obtained from healthy subjects and patients with known lung tumours has been challenged with fluorescent probes for the presence of an active cell surface protease. The mature epithelial cells from healthy patients' sputum lacked ability to bind these fluorescent probes whilst the majority of mature epithelial cells in the tumour patients' sputum bound these probes and consequently fluoresced. This demonstrable difference in the cell surface chemistry of mature epithelial cells was linked to the presence of lung tumour cells, which also possessed this cell surface protease. The mechanism of this induced cell surface enzyme appearance is not understood.
从健康受试者和已知患有肺部肿瘤的患者身上获取的痰液，已用荧光探针检测活性细胞表面蛋白酶的存在。健康患者痰液中的成熟上皮细胞缺乏结合这些荧光探针的能力，而肿瘤患者痰液中的大多数成熟上皮细胞则能结合这些探针并因此发出荧光。成熟上皮细胞在细胞表面化学性质上的这种明显差异与肺部肿瘤细胞的存在有关，肺部肿瘤细胞也具有这种细胞表面蛋白酶。这种诱导性细胞表面酶出现的机制尚不清楚。
