从衰老的人类成纤维细胞中分离出的DNA聚合酶活性保真度降低。
Decreased fidelity of DNA polymerase activity isolated from aging human fibroblasts.
作者信息
Linn S, Kairis M, Holliday R
出版信息
Proc Natl Acad Sci U S A. 1976 Aug;73(8):2818-22. doi: 10.1073/pnas.73.8.2818.
Abstract
DNA polymerase (deoxynucleosidetriphosphate: DNA nucleotidyltransferase, EC 2.7.7.7 or DNA nucleotidyltransferase) activity, isolated from late and early passage cells of the diploid human fibroblast line, MRC-5, was compared. The level of activity dropped with increasing passage. In addition, when the fidelity of polymerization was monitored with four synthetic templates under a variety of conditions, it was observed that the enzyme from late passage cells was more error-prone. The possible relation of these observations to "senescence" of the fibroblasts is discussed.
摘要
对从二倍体人成纤维细胞系MRC - 5的早期和晚期传代细胞中分离出的DNA聚合酶（脱氧核苷三磷酸：DNA核苷酸转移酶，EC 2.7.7.7或DNA核苷酸转移酶）活性进行了比较。活性水平随着传代次数的增加而下降。此外，在各种条件下用四种合成模板监测聚合保真度时，观察到晚期传代细胞中的酶更容易出错。讨论了这些观察结果与成纤维细胞“衰老”的可能关系。
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