Rehman I, Karsh M, Hench L L, Bonfield W
Interdisciplinary Research Centre in Biomedical Materials, Queen Mary & Westfield College, University of London, Mile End Road, London E1 4NS, United Kingdom.
J Biomed Mater Res. 2000 May;50(2):97-100. doi: 10.1002/(sici)1097-4636(200005)50:2<97::aid-jbm1>3.0.co;2-7.
A nucleation and crystallization schedule was adapted to produce 40% crystalline Bioglass ceramic particulates. These particles were placed in a dynamic environment in a simulated physiologic solution (SBF-9) for time periods ranging from 10 min to 7 days. Fourier transform Raman spectroscopy (FT-Raman) and infrared spectroscopy (FTIR) were used to analyze the apatite layer formation on the particulates. FTIR determined that amorphous apatite formation took place within 2 h, with the appearance of crystalline apatite in 14 h. The vibrational frequencies obtained through FT-Raman were equivalent to those obtained using FTIR. These analyses showed that a fully crystallized apatite layer was present on the particulate after 3 days of exposure in SBF solution. These findings are consistent with those associated with amorphous Bioglass particles.
采用成核和结晶程序制备40%结晶的生物玻璃陶瓷颗粒。将这些颗粒置于模拟生理溶液（SBF-9）的动态环境中，放置时间从10分钟到7天不等。利用傅里叶变换拉曼光谱（FT-Raman）和红外光谱（FTIR）分析颗粒上磷灰石层的形成情况。FTIR确定在2小时内形成了无定形磷灰石，14小时后出现结晶磷灰石。通过FT-Raman获得的振动频率与使用FTIR获得的频率相当。这些分析表明，在SBF溶液中暴露3天后，颗粒上存在完全结晶的磷灰石层。这些发现与无定形生物玻璃颗粒的相关发现一致。
