Reguera M J, Rabanal R M, Puigdemont A, Ferrer L
Department of Patologia i Producció Animals, Universitat Autònoma de Barcelona, Spain.
Am J Dermatopathol. 2000 Feb;22(1):49-54. doi: 10.1097/00000372-200002000-00010.
c-kit protooncogene encodes a type III transmembrane receptor kinase, the stem cell factor receptor, or KIT. The ligand of the KIT. stem cell factor, is a cytokine that stimulates mast cell growth and differentiation. We have studied immunohistochemically KIT expression in 23 canine mast cell tumors (MCTs), 10 histiocytomas, 5 malignant melanomas, and in 2 cell lines derived from mast cells (HMC-1, human and C2, canine). As expected, KIT was detected both in the human mast cell leukemia cell line (HMC- ) and in the canine mastocytoma cell line C2. In normal canine skin, KIT expression was confined to mast cells. All canine MCTs expressed KIT, although the intensity of the staining reaction varied considerably among the 23 neoplasms. Grade III tumors showed the highest expression of KIT, whereas grade I tumors showed the lowest expression of KIT. Two patterns of KIT expression were detected in mast cells. In normal canine mast cells and in some neoplastic mast cells, KIT appeared mainly on the cell membrane. However, in many canine MCTs, KIT is accumulated in the cytoplasm, usually near the cell nucleus. The meaning of these two patterns is not clear. Expression of KIT could not be detected immunohistochemically in any of the other neoplasias investigated. According to our results, it can be concluded that most, if not all, canine MCT express KIT. Furthermore, there is an inverse correlation between the degree of differentiation and the expression of KIT. Moreover, according to our results, KIT can be used as a reliable immunohistochemical marker for canine mast cells and undifferentiated mast cell tumors.
c-kit原癌基因编码一种III型跨膜受体激酶，即干细胞因子受体或KIT。KIT的配体——干细胞因子，是一种刺激肥大细胞生长和分化的细胞因子。我们采用免疫组织化学方法研究了23例犬肥大细胞瘤（MCT）、10例组织细胞瘤、5例恶性黑色素瘤以及2种源自肥大细胞的细胞系（人源HMC-1和犬源C2）中KIT的表达情况。正如预期的那样，在人肥大细胞白血病细胞系（HMC-1）和犬肥大细胞瘤细胞系C2中均检测到了KIT。在正常犬皮肤中，KIT表达局限于肥大细胞。所有犬MCT均表达KIT，尽管在这23个肿瘤中染色反应强度差异很大。III级肿瘤显示KIT表达最高，而I级肿瘤显示KIT表达最低。在肥大细胞中检测到两种KIT表达模式。在正常犬肥大细胞和一些肿瘤性肥大细胞中，KIT主要出现在细胞膜上。然而，在许多犬MCT中，KIT积聚在细胞质中，通常靠近细胞核。这两种模式的意义尚不清楚。在所研究的任何其他肿瘤中均未通过免疫组织化学检测到KIT的表达。根据我们的结果，可以得出结论，大多数（如果不是全部）犬MCT表达KIT。此外，分化程度与KIT表达之间存在负相关。而且，根据我们的结果，KIT可作为犬肥大细胞和未分化肥大细胞瘤的可靠免疫组织化学标志物。
