Koeppen K M, Boll I
Blut. 1978 Jun 20;36(6):347-351. doi: 10.1007/BF01000592.
Peripheral blood cells from patients with acute leukaemia were stimulated in RPMI-medium with Phytohaemagglutinin (PHA) and/or Pokeweed-Mitogen (PWM). Samples of the cultures were taken at 3, 5 and 7 day intervals for cytological characterization. From 119 cultures from 65 patients 76 cultures from 47 patients could be analysed. Cultures stimulated with PHA and/or PWM showed in 43,4% a higher stimulation rate in comparison with cultures from normal persons. These findings are discussed in relation to the increased agar-colony growth growth of leukaemic cells after in vitro PHA-stimulation and transformation of blast cells.
来自急性白血病患者的外周血细胞在含有植物血凝素（PHA）和/或商陆丝裂原（PWM）的RPMI培养基中进行刺激。每隔3、5和7天采集培养物样本进行细胞学特征分析。在65例患者的119份培养物中，有47例患者的76份培养物可以进行分析。与正常人的培养物相比，用PHA和/或PWM刺激的培养物中有43.4%显示出更高的刺激率。结合体外PHA刺激后白血病细胞琼脂集落生长增加以及原始细胞转化，对这些发现进行了讨论。
