Ten-Hage L, Delaunay N, Pichon V, Couté A, Puiseux-Dao S, Turquet J
Laboratoire de Cryptogamie, Muséum National d'Histoire Naturelle, 12, rue Buffon, 75231, Paris, France.
Toxicon. 2000 Aug;38(8):1043-54. doi: 10.1016/s0041-0101(99)00216-0.
Okadaic acid was isolated from a strain of Prorocentrum arenarium Faust (Prorocentrales, Dinophyceae) collected from Europa Island (40 degrees 22'E, 22 degrees 20'S, SW Indian Ocean). The presence of okadaic acid in the algal extract was suspected after cytotoxicity and phosphatase 2A inhibition testing. It was confirmed by ADAM derivatization, immunoaffinity extraction and liquid chromatography with fluorimetric detection analysis as well as by liquid microchromatography with mass spectrometric detection. Results indicate that the P. arenarium strain was toxinogenic and could be potentially involved in the toxin production associated with the human diseases, diarrhetic shellfish poisoning and possibly ciguatera fish poisoning in the SW Indian Ocean area.
冈田酸是从采自印度洋西南部欧罗巴岛（东经40度22分，南纬22度20分）的一株沙原甲藻（原甲藻目，甲藻纲）中分离得到的。在进行细胞毒性和磷酸酶2A抑制试验后，怀疑藻类提取物中存在冈田酸。通过ADAM衍生化、免疫亲和萃取、荧光检测液相色谱分析以及质谱检测液相微色谱法得以证实。结果表明，该沙原甲藻菌株具有产毒性，可能与印度洋西南部地区与人类疾病——腹泻性贝类中毒以及可能的雪卡毒素中毒相关的毒素产生有关。
