鉴定来自佛罗里达湾的海洋甲藻原甲藻中存在的冈田酸。
Identification of okadaic acid production in the marine dinoflagellate Prorocentrum rhathymum from Florida Bay.
机构信息
Department of Chemistry and Biochemistry, Florida International University, 11200 SW 8th St., Miami, FL 33199, USA.
出版信息
Toxicon. 2010 Feb-Mar;55(2-3):653-7. doi: 10.1016/j.toxicon.2009.08.018. Epub 2009 Sep 6.
Abstract
Extracts of fifty-seven newly isolated strains of dinoflagellates and raphidophytes were screened for protein phosphatase (PP2A) inhibition. Five strains, identified by rDNA sequence analysis as Prorocentrum rhathymum, tested positive and the presence of okadaic acid was confirmed in one strain by HPLC-MS/MS and by HPLC with fluorescence detection and HPLC-MS of the okadaic acid ADAM derivative. Quantitation of the ADAM derivative indicated that the concentration of okadaic acid in the culture medium is 0.153 microg/L.
摘要
对 57 株新分离的甲藻和腰鞭毛藻类的提取物进行了蛋白磷酸酶 (PP2A) 抑制的筛选。通过 rDNA 序列分析鉴定为原甲藻属的 5 株阳性菌株，并通过 HPLC-MS/MS 和 HPLC 荧光检测以及 ADAM 衍生物的 HPLC-MS 分析，在其中一株菌中证实了存在 okadaic acid。ADAM 衍生物的定量表明，培养基中 okadaic acid 的浓度为 0.153 微克/升。
相似文献
1
Identification of okadaic acid production in the marine dinoflagellate Prorocentrum rhathymum from Florida Bay.鉴定来自佛罗里达湾的海洋甲藻原甲藻中存在的冈田酸。
Toxicon. 2010 Feb-Mar;55(2-3):653-7. doi: 10.1016/j.toxicon.2009.08.018. Epub 2009 Sep 6.
2
Evidence of okadaic acid production in a cultured strain of the marine dinoflagellate Prorocentrum rhathymum from Malaysia.在马来西亚海洋甲藻原甲藻培养株中发现了冈田酸的产生。
Toxicon. 2010 Feb-Mar;55(2-3):633-7. doi: 10.1016/j.toxicon.2009.07.016. Epub 2009 Jul 23.
3
Okadaic acid production from the marine benthic dinoflagellate Prorocentrum arenarium Faust (Dinophyceae) isolated from Europa Island coral reef ecosystem (SW Indian Ocean).从印度洋西南部欧罗巴岛珊瑚礁生态系统分离出的海洋底栖甲藻阿氏原甲藻（Prorocentrum arenarium Faust，甲藻纲）产生冈田酸的研究。
Toxicon. 2000 Aug;38(8):1043-54. doi: 10.1016/s0041-0101(99)00216-0.
4
Polyketides from marine dinoflagellates of the genus Prorocentrum, biosynthetic origin and bioactivity of their okadaic acid analogues.原甲藻属海洋甲藻中的聚酮化合物，其 okadaic 酸类似物的生物合成来源和生物活性。
Mini Rev Med Chem. 2010 Jan;10(1):51-61. doi: 10.2174/138955710791112541.
5
Okadaic acid production from the marine dinoflagellate Prorocentrum belizeanum Faust isolated from the Belizean coral reef ecosystem.从伯利兹珊瑚礁生态系统分离出的海洋双鞭毛藻伯利兹原甲藻产生冈田酸。
Toxicon. 1998 Jan;36(1):201-6. doi: 10.1016/s0041-0101(97)00054-8.
6
Toxicity to medaka fish embryo development of okadaic acid and crude extracts of Prorocentrum dinoflagellates.冈田酸和原甲藻鞭毛虫粗提物对青鳉鱼胚胎发育的毒性
Toxicon. 2007 Jun 15;49(8):1182-92. doi: 10.1016/j.toxicon.2007.02.008. Epub 2007 Feb 24.
7
Diverse bacterial PKS sequences derived from okadaic acid-producing dinoflagellates.源自产生冈田酸的甲藻的多种细菌聚酮合酶序列。
Mar Drugs. 2008 May 22;6(2):164-79. doi: 10.3390/md20080009.
8
The toxic benthic dinoflagellate Prorocentrum maculosum Faust is a synonym of Prorocentrum hoffmannianum Faust.有毒底栖甲藻夜光藻 Faust 是 Faust 氏拟菱形藻的同义词。
Harmful Algae. 2018 Sep;78:1-8. doi: 10.1016/j.hal.2018.06.009. Epub 2018 Jul 11.
9
Identification of the okadaic acid-based toxin profile of a marine dinoflagellate strain Prorocentrum lima by LC-MS/MS and NMR spectroscopic data.采用 LC-MS/MS 和 NMR 波谱数据分析鉴定海洋甲藻品系 Prorocentrum lima 产生的冈田酸类毒素特征。
J Sep Sci. 2012 Apr;35(7):782-9. doi: 10.1002/jssc.201100914.
10
18O-Labelling pattern of okadaic acid from H218O in dinoflagellate Prorocentrum lima elucidated by tandem mass spectrometry.通过串联质谱法阐明利马原甲藻中由H218O产生的冈田酸的18O标记模式。
Eur J Biochem. 2000 Aug;267(16):5179-83. doi: 10.1046/j.1432-1327.2000.01586.x.
引用本文的文献
1
The Chemistry of Phytoplankton.浮游植物的化学
Chem Rev. 2024 Dec 11;124(23):13099-13177. doi: 10.1021/acs.chemrev.4c00177. Epub 2024 Nov 21.
2
Industrial Applications of Dinoflagellate Phycotoxins Based on Their Modes of Action: A Review.基于作用模式的甲藻藻毒素的工业应用：综述。
Toxins (Basel). 2020 Dec 18;12(12):805. doi: 10.3390/toxins12120805.
3
Effects of substratum and depth on benthic harmful dinoflagellate assemblages.底质和深度对底栖有害甲藻组合的影响。
Sci Rep. 2020 Jul 9;10(1):11251. doi: 10.1038/s41598-020-68136-6.
4
Degradation of okadaic acid in seawater by UV/TiO photocatalysis - Proof of concept.UV/TiO 光催化降解海水中的 okadaic 酸-概念验证。
Sci Total Environ. 2020 Sep 1;733:139346. doi: 10.1016/j.scitotenv.2020.139346. Epub 2020 May 12.
5
Fluorometric determination of okadaic acid using a truncated aptamer.荧光法测定断尾适体对冈田酸的识别
Mikrochim Acta. 2019 Jun 10;186(7):406. doi: 10.1007/s00604-019-3517-3.
6
Effect of Suspended Particulate Matter on the Accumulation of Dissolved Diarrhetic Shellfish Toxins by Mussels () under Laboratory Conditions.实验室条件下悬浮颗粒物对贝类蓄积麻痹性贝类毒素的影响()。
Toxins (Basel). 2018 Jul 3;10(7):273. doi: 10.3390/toxins10070273.
7
Mixtures of Lipophilic Phycotoxins: Exposure Data and Toxicological Assessment.脂溶性藻毒素混合物：暴露数据和毒理学评估。
Mar Drugs. 2018 Jan 31;16(2):46. doi: 10.3390/md16020046.
8
Detection of Dinophysistoxin-1 in Clonal Culture of Marine Dinoflagellate Prorocentrum foraminosum (Faust M.A., 1993) from the Sea of Japan.从日本海的海洋甲藻有孔前沟藻（Faust M.A., 1993）克隆培养物中检测出鳍藻毒素-1 。
Toxins (Basel). 2015 Sep 28;7(10):3947-59. doi: 10.3390/toxins7103947.
9
Okadaic acid meet and greet: an insight into detection methods, response strategies and genotoxic effects in marine invertebrates.冈田酸：海洋无脊椎动物的检测方法、应对策略和遗传毒性作用的深入了解。
Mar Drugs. 2013 Aug 9;11(8):2829-45. doi: 10.3390/md11082829.
10
The structures of three metabolites of the algal hepatotoxin okadaic acid produced by oxidation with human cytochrome P450.三种藻类肝毒素 okadaic 酸人细胞色素 P450 氧化产物的代谢物结构。
Bioorg Med Chem. 2012 Jun 15;20(12):3742-5. doi: 10.1016/j.bmc.2012.04.046. Epub 2012 Apr 28.
本文引用的文献
1
PROROCENTRUM LEVIS, A NEW BENTHIC SPECIES (DINOPHYCEAE) FROM A MANGROVE ISLAND, TWIN CAYS, BELIZE(1).浅平扁藻，一种来自伯利兹双礁岛红树林岛的新底栖物种（甲藻纲）(1) 。
J Phycol. 2008 Feb;44(1):232-40. doi: 10.1111/j.1529-8817.2007.00450.x.
2
Viable cell sorting of dinoflagellates by multiparametric flow cytometry.利用多参数流式细胞术对甲藻进行活细胞分选。
Phycologia. 2009 Jul 1;48(4):249-257. doi: 10.2216/08-51.1.
3
Multiple simultaneous detection of Harmful Algal Blooms (HABs) through a high throughput bead array technology, with potential use in phytoplankton community analysis.通过高通量微珠阵列技术同时对有害藻华进行多重检测，该技术在浮游植物群落分析中具有潜在应用价值。
Harmful Algae. 2009;8(2):196-211. doi: 10.1016/j.hal.2008.05.003.
4
Evidence of okadaic acid production in a cultured strain of the marine dinoflagellate Prorocentrum rhathymum from Malaysia.在马来西亚海洋甲藻原甲藻培养株中发现了冈田酸的产生。
Toxicon. 2010 Feb-Mar;55(2-3):633-7. doi: 10.1016/j.toxicon.2009.07.016. Epub 2009 Jul 23.
5
Diverse bacterial PKS sequences derived from okadaic acid-producing dinoflagellates.源自产生冈田酸的甲藻的多种细菌聚酮合酶序列。
Mar Drugs. 2008 May 22;6(2):164-79. doi: 10.3390/md20080009.
6
Characterization of fatty acid esters of okadaic acid and related toxins in blue mussels (Mytilus edulis) from Norway.挪威蓝贻贝（紫贻贝）中冈田酸及相关毒素的脂肪酸酯的特性分析
Rapid Commun Mass Spectrom. 2008 Apr;22(8):1127-36. doi: 10.1002/rcm.3490.
7
Extraction of microalgal toxins by large-scale pumping of seawater in Spain and Norway, and isolation of okadaic acid and dinophysistoxin-2.在西班牙和挪威通过大规模抽取海水提取微藻毒素，并分离出冈田酸和鳍藻毒素-2。
Toxicon. 2007 Dec 1;50(7):960-70. doi: 10.1016/j.toxicon.2007.07.003. Epub 2007 Jul 27.
8
Systematics of basidiomycetous yeasts: a comparison of large subunit D1/D2 and internal transcribed spacer rDNA regions.担子菌酵母的系统学：大亚基D1/D2和内部转录间隔区rDNA区域的比较
FEMS Yeast Res. 2002 Dec;2(4):495-517. doi: 10.1111/j.1567-1364.2002.tb00117.x.
9
Okadaic acid: the archetypal serine/threonine protein phosphatase inhibitor.冈田酸：典型的丝氨酸/苏氨酸蛋白磷酸酶抑制剂。
Curr Med Chem. 2002 Nov;9(22):1939-80. doi: 10.2174/0929867023368791.
10
Okadaic acid production from the marine benthic dinoflagellate Prorocentrum arenarium Faust (Dinophyceae) isolated from Europa Island coral reef ecosystem (SW Indian Ocean).从印度洋西南部欧罗巴岛珊瑚礁生态系统分离出的海洋底栖甲藻阿氏原甲藻（Prorocentrum arenarium Faust，甲藻纲）产生冈田酸的研究。
Toxicon. 2000 Aug;38(8):1043-54. doi: 10.1016/s0041-0101(99)00216-0.
Zotero 插件