Anselme K
Institut de Recherche sur les Maladies du Squelette, Institut Calot, Berck sur mer, France.
Biomaterials. 2000 Apr;21(7):667-81. doi: 10.1016/s0142-9612(99)00242-2.
The development of tissue engineering in the field of orthopaedic surgery is now booming. Two fields of research in particular are emerging: the association of osteo-inductive factors with implantable materials; and the association of osteogenic stem cells with these materials (hybrid materials). In both cases, an understanding of the phenomena of cell adhesion and, in particular, understanding of the proteins involved in osteoblast adhesion on contact with the materials is of crucial importance. The proteins involved in osteoblast adhesion are described in this review (extracellular matrix proteins, cytoskeletal proteins, integrins, cadherins, etc.). During osteoblast/material interactions, their expression is modified according to the surface characteristics of materials. Their involvement in osteoblastic response to mechanical stimulation highlights the significance of taking them into consideration during development of future biomaterials. Finally, an understanding of the proteins involved in osteoblast adhesion opens up new possibilities for the grafting of these proteins (or synthesized peptide) onto vector materials, to increase their in vivo bioactivity or to promote cell integration within the vector material during the development of hybrid materials.
目前，骨外科领域的组织工程发展蓬勃。尤其出现了两个研究领域：骨诱导因子与可植入材料的结合；成骨干细胞与这些材料（混合材料）的结合。在这两种情况下，了解细胞粘附现象，特别是了解与材料接触时参与成骨细胞粘附的蛋白质至关重要。本综述描述了参与成骨细胞粘附的蛋白质（细胞外基质蛋白、细胞骨架蛋白、整合素、钙粘蛋白等）。在成骨细胞/材料相互作用过程中，它们的表达会根据材料的表面特性而改变。它们参与成骨细胞对机械刺激的反应，凸显了在未来生物材料开发过程中考虑它们的重要性。最后，了解参与成骨细胞粘附的蛋白质为将这些蛋白质（或合成肽）接枝到载体材料上开辟了新的可能性，以提高其体内生物活性或在混合材料开发过程中促进细胞在载体材料内的整合。
