Seale T W, Thirkill H, Tarpay M, Flux M, Rennert O M
J Clin Microbiol. 1979 Jan;9(1):72-8. doi: 10.1128/jcm.9.1.72-78.1979.
A phenotypic characterization of Pseudomonas aeruginosa from single sputum samples of 21 typical cystic fibrosis patients indicated a high frequency of heterogeneity among isolates on the basis of differences in antibiotic resistance, colony morphology, pigmentation, and serotype. Two or more isolates with different but stable susceptibilities to carbenicillin, gentamycin, streptomycin, tetracycline, chloramphenicol, and sulfamethoxazole plus trimethoprim were detected in 38% of the sputa. Differences generally were independent of the mucoid state of the strain. O-antigen group determination with the Difco typing set showed that two or more serologically distinct strains were present in 10/21 sputum specimens. Nonmucoid derivatives of mucoid isolates almost always retained both the antibiotic susceptibilities and serotype of their parent strain. These data suggest that cystic fibrosis patients may be cocolonized/coinfected by different strains of P. aeruginosa more frequently than generally believed. Alternatively, phenotypically distinct strains from a single patient might arise as phenotypic dissociants from a single infecting strain. Because of the frequency and multiplicity of phenotypically distinct P. aeruginosa isolates which we obtained from our cystic fibrosis patients, it is important to select multiple isolates from sputum cultures for antimicrobial susceptibility testing so as to assess adequately the susceptibility of this organism to antibiotic therapy in cystic fibrosis. We recommend that several colonies of each distinguishable colony type of P. aeruginosa be pooled for the antibiogram.
对21例典型囊性纤维化患者的单份痰液样本中的铜绿假单胞菌进行表型特征分析，结果表明，基于抗生素耐药性、菌落形态、色素沉着和血清型的差异，分离株之间存在高度异质性。在38%的痰液中检测到两种或更多种对羧苄青霉素、庆大霉素、链霉素、四环素、氯霉素以及磺胺甲恶唑加甲氧苄啶具有不同但稳定敏感性的分离株。这些差异通常与菌株的黏液样状态无关。使用Difco分型试剂盒进行O抗原组测定显示，在10/21份痰液标本中存在两种或更多种血清学上不同的菌株。黏液样分离株的非黏液样衍生物几乎总是保留其亲本菌株的抗生素敏感性和血清型。这些数据表明，囊性纤维化患者可能比一般认为的更频繁地被不同菌株的铜绿假单胞菌共同定植/合并感染。或者，来自单一患者的表型不同的菌株可能是单一感染菌株的表型解离产物。由于我们从囊性纤维化患者中获得的表型不同的铜绿假单胞菌分离株的频率和多样性，从痰液培养物中选择多个分离株进行抗菌药物敏感性测试很重要，以便充分评估该生物体对囊性纤维化中抗生素治疗的敏感性。我们建议将铜绿假单胞菌每种可区分菌落类型的几个菌落合并用于抗菌谱分析。
